Nitric Oxide Cell-Based HTS Assay Kit

• Catalog number
  K979-100
• Price
  Please ask
• Size
  100 assays

• Description
  Ultrasensitive, Simple, HTP adaptable fluorometric assay kit for measuring NO concentration
• Summary
  Ultrasensitive, Simple, HTP adaptable fluoromotericassay kit for concentration of nitric oxide. Can detect as low as 5 pmol of Nitric Oxide concentration Detection method- Fluorometric analysis (Ex/Em = 488/532 nm) Applications- Detection of nitric concentration concentrations in cells Sample Type: adherent or suspension cells
• Detection Method
  Fluorometric analysis (Ex/Em = 488/532 nm)
• Species Reactivity
  Mammalian cell culture
• Applications
  Detection of concentration of nitric oxide
• Sample Type
  Adherent or suspension cells
• Features Benefits
  Simple, HTP adaptable protocol Sensitivity as low as 5 pmol of nitric oxide in cells Fluorometric detection
• Storage Conditions
  -20°C
• Shipping Conditions
  gel pack
• Shelf life
  12 months
• Background
  Nitric oxide (NO) plays an important role in neurotransmission, vascular regulation, immune response and apoptosis. NO has been established as one of the key regulators in cardiovascular, nervous, and immune systems. NO plays a pivotal role in numerous processes: in central nervous system, NO participates in cell communication and information storage; in vascular endothelium, NO is involved with regulation of vascular function. Finally, NO is produced by immune cells, including macrophages, as a part of body’s defense mechanism during immunological responses and oxidative stress conditions. However, chronic overproduction of NO is one of the fundamental causes underlying disorders including neurodegenerative diseases and pathophysiology of blood vessels. BioVision’s Nitric Oxide Cell-Based HTS Assay Kit utilizes a dye that reacts with intracellular NO to produce a highly-Fluorescent Triazole Product (Ex/Em= 488/532 nm). The kit includes Diphenyleneiodonium (DPI), a potent inhibitor of nitric oxide cellular production, which has been widely used to inhibit NO synthesis in order to evaluate NO function in different systems and diseases. This kit provides a simple, standardized and HTS method to quantitate the amount of NO in cell-based assays. This Assay Kit is user-friendly, sensitive and can detect the NO Fluorescent product as low as 5 pmol in a 96-well plate
• Data Sheet Link
• Test
  Biovision supplies other types of Assays as 1.
• Additional description
  For cells, cell lines and tissues in culture till half confluency.

• Gene target
  Nitric   Oxide   Based   HTS   Kit  
• Short name
  Nitric Oxide -Based HTS Assay Kit
• Technique
  Assay, HTS, assays
• Host
  Assay
• Alternative name
  Nitric Oxide cellular-Based HTS test reagent
• Alternative technique
  arrays, kits
• Alternative to gene target
  v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, transferase activity, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832
• Tissue
  cell

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