MAPKAPK2, Active

Synonyms

MAPKAPK, MAP kinase-activated protein kinase 2
Alternative_names

MAPKAPK, MAP kinase-activated protein kinase 2
Description

Involved in many signaling cascades
Recombinant

Yes
Source

Baculovirus (Sf9 insect cells)
Purity by SDS PAGE

≥90%
Assay

SDS-PAGE
Activity Specifications test method

254 nmol phosphate incorporated into MAPKAP kinase 2 substrate peptide per minute per mg protein at 30°C for 15 minutes using a final concentration of 50 µM ATP and total of 0.83 µCi/µl P-32.
Molecular Weight

68.0 kDa
Storage Temp

-80°C
Shipping

dry ice
Shelf Life

12 months
Concentration

5µg/50 µl
Appearance

Liquid
Physical form description

Recombinant protein in storage buffer (50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 0.25 mM DTT, 0.1 mM EGTA, 0.1 mM EDTA, 0.1 mM PMSF, 25% glycerol).
Background Information

MAPKAP kinase2 is a Ser/Thr protein kinase, which is regulated through direct phosphorylation by p38 MAP kinase. In conjunction with p38 MAP kinase, this kinase is known to be involved in many cellular processes including stress and inflammatory responses, nuclear export, gene expression regulation and cell proliferation. Heat shock protein HSP27 was shown to be one of the substrates of this kinase in vivo.
Handling

Centrifuge the vial prior to opening.
Usage

For Research Use Only! Not to be used in humans
Datasheet

Identity

HGNC:6887
Gene

MAPKAPK2
Long gene name

MAPK activated protein kinase 2
Synonyms gene name
- mitogen-activated protein kinase-activated protein kinase 2
Synonyms
- MK2
GenBank acession
- U12779
Locus

1q32.1
Discovery year

1999-03-26
Entrez gene record

9261
Pubmed identfication
- 8179591
- 8280084
RefSeq identity
- NM_004759
Classification
- MAPK activated protein kinases
VEGA ID

OTTHUMG00000036342