Lysosome Purification Kit

• Catalog number
  K235-50
• Price
  Please ask
• Size
  50 Extractions

• Description
  Simple method to isolate enriched or purified lysosomes from tissue and cultured cells
• Summary
  Applications: • Isolation of high purity lysosomes from tissues and cultured cells • Lysosome studies like enzyme activity or uptake studies • Protein profiling of lysosomes by SDS-PAGE and Western blot
• Applications
  • Isolation of intact functional lysosomes from tissues and cultured cells • Lysosome studies like enzyme activity or uptake studies • Protein profiling of Lysosomes by SDS-PAGE and Western blot
• Sample Type
  • Mammalian glands and soft tissues such as liver, kidney, etc. • Cultured eukaryotic cell lines
• Features Benefits
  • Simple & convenient protocol • The kit provides unique formulations of ready-to-use buffers and reagents to isolate lysosomes with minimal sample preparation
• Storage Conditions
  -20°C
• Shipping Conditions
  gel pack
• Shelf life
  12 months
• Background
  Lysosomes are membrane-bound cell organelles found in most animal cells (except red blood cells). Lysosomes are spherical vesicles containing hydrolytic enzymes capable of breaking down different kinds of biomolecules, including proteins, nucleic acids, carbohydrates, lipids, and cellular debris. They are known to contain over 50 different enzymes, which are active under acidic conditions. Thus, lysosomes act as the waste disposal system of the cells by digesting unwanted materials in the cytoplasm. BioVision’s Lysosome Isolation Kit provides a procedure for isolating enriched or purified lysosomal fraction from animal tissues and cultured cells by differential centrifugation followed by density gradient centrifugation
• Data Sheet Link
• Test
  Antibodies are affinity purified with an antigen coated column or protein A or G agarose or beads. DNA is purified with endotoxin free silica columns or anion exchange resins. Biovision supplies purification kits and ultra pure reagents.

• Gene target
  Lysosome   Kit  
• Short name
  Lysosome Purification Kit
• Technique
  Purification, purified
• Alternative name
  Lysosome purification reagent
• Alternative technique
  purifications, kits
• Alternative to gene target
  v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, transferase activity, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832
• Tissue
  lysosome

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