Human Whole Blood Monocyte Isolation Kit

• Catalog number
  K933-30
• Price
  Please ask
• Size
  30 ml isolation

• Description
  Simple, one step assay to isolate monocytes
• Summary
  • Isolation of Monocytes from human whole blood. • Determination of cell viability and also contamination from RBCs in isolated cell fraction.
• Species Reactivity
  Human
• Applications
  • Isolation of Monocytess from human whole blood. • Determination of cell viability and also contamination from RBCs in isolated cell fraction.
• Sample Type
  Human whole blood (collected within 12 hr)
• Features Benefits
  • PBMC high yields (≥5 x106 cells/ml) • By using the Viability Stain, it was proven that 99% of the cells are viable and the isolated fraction contains low red blood cell counts (≤ 3%)
• Storage Conditions
  4 °C
• Shipping Conditions
  gel pack
• Shelf life
  12 months
• Background
  Monocytes are a type of white blood cell (leukocyte) and the largest of the immune cells. They are produced in the bone marrow and then migrate to the blood stream where they comprise 3-8% of all leukocytes. Depending on the clincial presentation of disease, monocytes will migrate to a specific tissue and then differentiate into a tissue-specific dendritic cell or a macrophage. Monocytes are easily identified by the large amoeboid shape, unilobar nucleus and vacuolated cytoplasm. They serve three important roles: phagocytosis, presentation of antigen to T cells which subsequently results in T cell clonal expansion, and the production of cytokines. BioVision’s human Whole Blood Monocyte Isolation Kit allows for in vitro isolation of intact, viable monocytes. The kit enables the isolation of ≥ 5 x106 monocytes/ml yielding approximately 80% of the total monocytes present in 1 ml of whole blood. The viability stain (included) used to identify living monocytes indicates that more than 99% of the cells in the isolated fraction are viable. The isolated fraction is pure - it contains more than 95% monocytes with less than 3% red blood cell counts.
• Data Sheet Link
• Additional description
  Blood is slightly basic and contains proteins, hemoglobin, albumins in its serum. Biovision supplies this blood related product in 1.
• Properties
  Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.

• Gene target
  Whole   Monocyte   Isolation   Kit  
• Short name
  Whole Blood Monocyte Isolation Kit
• Technique
  blood, blood samples
• Species
  Human, Humans
• Alternative name
  H. sapiens Whole Blood Monocyte purification reagent
• Alternative technique
  sera, kits
• Alternative to gene target
  v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, transferase activity, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832
• Tissue
  blood, whole

Product images

Similar products