Human CellExp™ CD28, Human/ Cynomolgus / Rhesus macaque Recombinant

  • Catalog number
    P1471-10
  • Price
    Please ask
  • Size
    10 µg
  • Synonyms
    T-cell-specific surface glycoprotein CD28, CD antigen: TP44, Tp44, T-Cell-Specific Surface Glycoprotein, CD28 Molecule
  • Alternative_names
    T-cell-specific surface glycoprotein CD28, CD antigen: TP44, Tp44, T-Cell-Specific Surface Glycoprotein, CD28 Molecule
  • Description
    T-cell-specific surface glycoprotein CD28
  • Recombinant
    True
  • Source
    HEK 293 cells
  • Purity by SDS PAGE
    >90%
  • Assay
    SDS-PAGE
  • Biological activity
    Human biotinylated CD28 can bind to immobilized human CD86 (BioVision Cat# 7496 at 5 ug/ml, 100 ul/well) with a linear detection range of 0.08 to 0.6 ug/ml.
  • Molecular Weight
    This protein is fused with a polyhistidine tag at the C-terminus with the calculated molecular weight of ~16 kDa. The predicted N-terminus is Asn 19 (UniProtKB – P10747 CD28_HUMAN). DTT-reduced protein shows a band about 35-45 kDa due to heavy glycosylation.
  • Storage Temp
    -20°C
  • Shipping
    Gel Pack
  • Shelf Life
    12 months
  • Appearance
    Lyophilized powder
  • Physical form description
    Lyophilized
  • Reconstitution Instructions
    Centrifuge the vial prior to opening. Reconstitute in PBS, pH 7.4.
  • Background Information
    Human CD28 (Cluster of Differentiation 28) is expressed on the cell surface as a glycosylated, disulfide-linked homodimer. CD28 is the founding member of a subfamily of costimulatory molecules characterized by an extracellular variable immunoglobulin-like domain. Other members of the subfamily include ICOS, CTLA4, PD1, PD1H, and BTLA. CD28 and CTLA4 are highly homologous and compete for the same ligands B7-1 (CD80) and B7-2 (CD86). CD28 is expressed constitutively on almost all human CD4 T cells and approximately 50% of CD8 T cells. CD28 has diverse effects on T cell functions including the induction of cell proliferation and cytokine production and promotion of T-cell survival. Experiments showed that CD28 and CTLA4 have opposing effects on T cell stimulation. CD28 provides an activating signal and CTLA4 provides an inhibitory signal. Translation of a basic understanding of CD28 function into immunomodulatory therapeutics has been uneven, with both successes and failures from pre-clinical studies.
  • Amino acid sequence
    Asn 19 - Pro 152
  • Handling
    Centrifuge the vial prior to opening.
  • Properties
    Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
  • Additional source
    Recombinants or rec. proteins
  • Group
    recombinants
  • About
    Monkeys like rhesus's or the rhesus macaque (Macaca mulatta) are the most used apes in laboraty models.
  • Gene target
  • Gene symbol
    CD28
  • Short name
    CellExp™ CD28, / Cynomolgus / macaque Recombinant
  • Technique
    Recombinant, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. Biovision advises they will be reconstituted in a buffer soluion or culture medium for cell culture.
  • Species
    Humans, Rhesuses
  • Alternative name
    H. sapiens CellExp™ CD28 molecule, H. sapiens/ Cynomolgus / Rhesus macaque Rec.
  • Alternative technique
    rec
  • Alternative to gene target
    CD28 molecule, Tp44, CD28 and IDBG-79183 and ENSG00000178562 and 940, identical protein binding, Cell surfaces, Cd28 and IDBG-159608 and ENSMUSG00000026012 and 12487, CD28 and IDBG-643371 and ENSBTAG00000007445 and 281050
Gene info
MeSH Data
  • Name
  • Concept
    Scope note: The initial culturing of cells derived directly from fresh TISSUES.
  • Tree numbers
    • E01.370.225.500.223.500
    • E05.200.500.265.500
    • E05.242.223.500
    • E05.481.500.249.500
  • Qualifiers
    ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
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