H3K4me2 polyclonal antibody

  • Catalog number
    6865-25
  • Price
    314.66 USD
  • Size
    25 μg
  • Antibody Target
    H3K4me2
  • Target Alternative Name
    Histone 3
  • Antibody Type
    Polyclonal
  • Species Reactivities
    Human.
  • Immunogen Sequence
    Polyclonal antibody raised in rabbit against histone H3 containing the dimethylated lysine 4 (H3K4me2), using a KLH-conjugated synthetic peptide.
  • Accession
    P68431
  • Gene Id
    8350
  • Appearance
    Colorless liquid
  • Form
    Liquid
  • Formulation
    In PBS with 0.05% sodium azide and 0.05% ProClin 300.
  • Purification
    Affinity Purified
  • Application
    Western blot, IF, ELISA, Dot Blot, ChIP.
  • Positive Control
    Western blot: HeLa cells, ELISA: Antigen, ChIP: HeLa cells, Dot blot: Histone Peptides.
  • Application And Usages
    WB: 1:1000, ELISA: 1:500, Dot Blot: 1:20,000, ChIP: 1 µl/ChIP, IF: 1:5000.
  • Description
    Histones are the main constituents of the protein part of chromosomes of eukaryotic cells. They are rich in the amino acids arginine and lysine and have been greatly conserved during evolution. Histones pack the DNA into tight masses of chromatin. Two core histones of each class H2A, H2B, H3 and H4 assemble and are wrapped by 146 base pairs of DNA to form one octameric nucleosome. Histone tails undergo numerous post-translational modifications, which either directly or indirectly alter chromatin structure to facilitate transcriptional activation or repression or other nuclear processes. In addition to the genetic code, combinations of the different histone modifications reveal the so-called “histone code”. Histone methylation and demethylation is dynamically regulated by respectively histone methyl transferases and histone demethylases.
  • Storage Condition
    -20 °C
  • Shipping Condition
    gel pack
  • Shelf Life
    12 months
  • Usage
    For Research Use Only! Not to be used in humans.
  • Handling
    The antibody solution should be gently mixed before use.
  • Western Blot Verified
    Verified
  • Immunocytochemistry Verified
    Not Verified
  • Immunofluorescence Verified
    Verified
  • Immunoprecipitation Verified
    Not Verified
  • ELISA Verified
    Verified
  • ChIP Verified
    Verified
  • Dot Blot Verified
    Verified
  • Flow Cytometry Verified
    Not Verified
  • Properties
    If you buy Antibodies supplied by Biovision they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
  • Group
    Polyclonals and antibodies
  • About
    Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera.
  • French translation
    anticorps
  • Gene target
    H3K4me2  
  • Short name
    H3K4me2 polyclonal antibody
  • Technique
    Polyclonal, Antibody, antibodies against human proteins, antibodies for, Polyclonal antibodies (pAbs) are mostly rabbit or goat antibodies that are secreted by different B cells, whereas monoclonal antibodies come from a single N cell lineage. Pabs are a collection of immunoglobulin molecules that react against a specific antigen, each identifying a different epitope.
  • Isotype
    Rabbit IgG
  • Alternative name
    H3K4me2 polyclonal (antibody to-)
  • Alternative technique
    polyclonals, antibodies
MeSH Data
  • Name
  • Concept
    Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
  • Tree numbers
    • E05.196.401.143
    • E05.301.300.096
    • E05.478.566.320.200
    • E05.601.262
    • E05.601.470.320.200
  • Qualifiers
    ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
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