Glutamate Decarboxylase α (GAD α), E. coli Recombinant
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Catalog number
P1404-50
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Price
Please ask
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Size
50 µU
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Synonyms
GAD-alpha, gadA, gadS
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Alternative_names
GAD-alpha, gadA, gadS
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Description
Converts glutamate to gamma-aminobutyrate (GABA)
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Recombinant
True
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Source
E. coli
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Purity by SDS PAGE
>95%
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Assay
SDS-PAGE
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Activity Specifications test method
This enzyme has a specific activity of ≥ 1 mU/mg based on its ability to convert glutamate to GABA, which can be detected fluorometrically at Ex/Em=535/595.
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Results
1 mU/mg
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Unit Definition
One unit is the amount of enzyme that will hydrolyze 1.0 µmole of glutamate per minute at pH 8 and 37 ̊C.
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Molecular Weight
53.6 kDa with C-terminal 6X-His tag
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Storage Temp
-20°C
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Shipping
Gel Pack
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Shelf Life
12 months
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Appearance
Lyophilized powder
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Physical form description
Lyophilized
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Reconstitution Instructions
Reconstitute the lyophilized protein in 10% glycerol with 5 mM 2-Mercaptoethanol to a final concentration of 1.0 mU/ml and incubate the reconstituted protein at 25 °C for 15 minutes.
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Background Information
Glutamate Decarboxylase α is a pyridoxal 5′-phosphate dependent enzyme. GAD α catalyzes conversion of glutamate to GABA. Aberrant expressions of GAD α have been associated with diabetes and neurodegenerative diseases such as Alzheimers and Parkinson’s Disease.
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Handling
Centrifuge the vial prior to opening.
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Additional description
E. coli recombinant proteins are affinity purified and supplied lyophilized. For human research only.
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Additional source
Recombinants or rec. proteins
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Group
recombinants
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Gene target
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Gene symbol
GAD1
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Short name
Glutamate Decarboxylase α (GAD α), Recombinant
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Technique
coli recombinant, Recombinant, from e. Coli, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. Biovision advises they will be reconstituted in a buffer soluion or culture medium for cell culture.
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Host
e. coli, Escherichia coli, Recombination of bioactive proteins and longer peptides in Escherichia Coli is done often with His tagging. Biovision supplies Rec. E. Coli affinity purified or tag purified antigens in 1 quantities or bulk volumes on request.
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Species
E. coli
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Alternative name
Glutamate Decarboxylase α (GAD α), E. coli Rec.
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Alternative technique
rec, escherichia
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Gene info
MeSH Data
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Name
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Concept
Scope note:
The initial culturing of cells derived directly from fresh TISSUES.
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Tree numbers
- E01.370.225.500.223.500
- E05.200.500.265.500
- E05.242.223.500
- E05.481.500.249.500
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Qualifiers
ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
Product images
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