Glutamate Decarboxylase α (GAD α), E. coli Recombinant

  • Catalog number
    P1404-50
  • Price
    Please ask
  • Size
    50 µU
  • Synonyms
    GAD-alpha, gadA, gadS
  • Alternative_names
    GAD-alpha, gadA, gadS
  • Description
    Converts glutamate to gamma-aminobutyrate (GABA)
  • Recombinant
    True
  • Source
    E. coli
  • Purity by SDS PAGE
    >95%
  • Assay
    SDS-PAGE
  • Activity Specifications test method
    This enzyme has a specific activity of ≥ 1 mU/mg based on its ability to convert glutamate to GABA, which can be detected fluorometrically at Ex/Em=535/595.
  • Results
    1 mU/mg
  • Unit Definition
    One unit is the amount of enzyme that will hydrolyze 1.0 µmole of glutamate per minute at pH 8 and 37 ̊C.
  • Molecular Weight
    53.6 kDa with C-terminal 6X-His tag
  • Storage Temp
    -20°C
  • Shipping
    Gel Pack
  • Shelf Life
    12 months
  • Appearance
    Lyophilized powder
  • Physical form description
    Lyophilized
  • Reconstitution Instructions
    Reconstitute the lyophilized protein in 10% glycerol with 5 mM 2-Mercaptoethanol to a final concentration of 1.0 mU/ml and incubate the reconstituted protein at 25 °C for 15 minutes.
  • Background Information
    Glutamate Decarboxylase α is a pyridoxal 5′-phosphate dependent enzyme. GAD α catalyzes conversion of glutamate to GABA. Aberrant expressions of GAD α have been associated with diabetes and neurodegenerative diseases such as Alzheimers and Parkinson’s Disease.
  • Handling
    Centrifuge the vial prior to opening.
  • Additional description
    E. coli recombinant proteins are affinity purified and supplied lyophilized. For human research only.
  • Additional source
    Recombinants or rec. proteins
  • Group
    recombinants
  • Gene target
  • Gene symbol
    GAD1
  • Short name
    Glutamate Decarboxylase α (GAD α), Recombinant
  • Technique
    coli recombinant, Recombinant, from e. Coli, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. Biovision advises they will be reconstituted in a buffer soluion or culture medium for cell culture.
  • Host
    e. coli, Escherichia coli, Recombination of bioactive proteins and longer peptides in Escherichia Coli is done often with His tagging. Biovision supplies Rec. E. Coli affinity purified or tag purified antigens in 1 quantities or bulk volumes on request.
  • Species
    E. coli
  • Alternative name
    Glutamate Decarboxylase α (GAD α), E. coli Rec.
  • Alternative technique
    rec, escherichia
Gene info
  • Identity
  • Gene
  • Long gene name
    glutamate decarboxylase 1
  • Synonyms gene
  • Synonyms gene name
    • glutamate decarboxylase 1 (brain, 67kD)
    • glutamate decarboxylase 1 (brain, 67kDa)
  • Locus
  • Discovery year
    1986-01-01
  • Entrez gene record
  • Pubmed identfication
  • VEGA ID
MeSH Data
  • Name
  • Concept
    Scope note: The initial culturing of cells derived directly from fresh TISSUES.
  • Tree numbers
    • E01.370.225.500.223.500
    • E05.200.500.265.500
    • E05.242.223.500
    • E05.481.500.249.500
  • Qualifiers
    ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
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