EZCell™ CFDA SE Cell Tracer Kit

• Catalog number
  K2057-200
• Price
  Please ask
• Size
  200 assays

• Description
  Flow cytometry based assay to determine cell proliferation using CFDA SE dye
• Summary
  BioVision’s EZCell™ CFDA SE Cell Tracer Kit provides an easy, rapid and sensitive way to determine cell proliferation using CFDA SE dye. The dye can be inherited by the daughter cells for up to 8 generations. Following each cell division, the fluorescence intensity is halved, which can be quantified by measuring the fluorescence intensity at Ex/Em = 492/517 nm. The assay is non-radioactive, accurate and can be used for cell tracing studies.
• Detection Method
  Flow Cytometry
• Species Reactivity
  All
• Applications
  Assessment of drugs that affect cell division,Tracing cell division in vitro and in vivo,Monitoring cell proliferation
• Sample Type
  Adherent or suspension cells
• Features Benefits
  The assay is non-radioactive, accurate and can be used for cell tracing studies.,An easy, rapid and sensitive way to determine cell proliferation using CFDA SE dye.
• Storage Conditions
  -20°C
• Shipping Conditions
  Gel Pack
• Shelf life
  12 months
• Background
  Carboxyfluorescein Diacetate Succinimidyl Ester (CFDA SE) is a colorless, non-fluorescent, stable cell-permeable dye. Once it enters into the cells, the intracellular esterases remove the acetate groups to generate Carboxyfluorescein Succinimidyl Ester, which then covalently binds to lysine residues or other amines and produces a bright fluorescent conjugate. The fluorescent conjugate is stable and is retained inside the cells during cell division and hence it is an ideal tool for cell tracing studies, both in vitro and in vivo. The EZCell™ CFDA SE Cell Tracer Kit provides an easy, rapid and sensitive way to determine cell proliferation using CFDA SE dye. The dye can be inherited by the daughter cells for up to 8 generations. Following each cell division, the fluorescence intensity is halved, which can be quantified by measuring the fluorescence intensity at Ex/Em = 492/517 nm. The assay is non-radioactive, accurate and can be used for cell tracing studies.
• Data Sheet Link
• Additional description
  For cells, cell lines and tissues in culture till half confluency.

• Gene target
  EZCell™   CFDA   Tracer   Kit  
• Short name
  EZCell™ CFDA SE Tracer Kit
• Alternative name
  EZCell™ CFDA SE cellular Tracer reagent
• Alternative technique
  kits
• Alternative to gene target
  v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, transferase activity, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832
• Tissue
  cell

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