Endothelin Converting Enzyme 1 Activity Assay Kit (Fluorometric)

  • Catalog number
    K536-100
  • Price
    Please ask
  • Size
    100 assays
  • Description
    Industry's first kit to detect ECE1 activity in biological samples
  • Summary
    • Detection method - Fluorescence (Ex/Em = 320/420 nm) • Applications- Detection of ECE1 activity in tissue/cell lysates/protein preparations
  • Detection Method
    Fluorometric (Ex/Em = 320/420 nm)
  • Species Reactivity
    Mammalian
  • Applications
    Detection of ECE1 activity in tissue/cell lysates
  • Sample Type
    • Mammalian tissues: lung, heart, kidney • Cell culture: HeLa Cell lysates
  • Features Benefits
    • Simple one-step reaction • Takes only 1-2 hrs • Non-radiometric fluorescent detection • HTP adaptable
  • Storage Conditions
    -20°C
  • Shipping Conditions
    gel pack
  • Shelf life
    12 months
  • Background
    Endothelin Converting Enzyme 1 (ECE1; EC: 3.4.24.71) is a membrane-bound metalloprotease that cleaves inactive big endothelin (big ET-1), which is the precursor of active endothelin. Studies have shown that big ET-1 could be used as a predictor of survival in patients suffering esophageal squamous cell carcinoma. ECE-1 is abundantly expressed in vivo in endothelial cells producing mature ET-1. As of today, Endothelin is the most potent vasoconstrictor known and it has cytokine- or hormone-like activities. Therefore, ECE-1 could play a significant role in pathogenesis of cardiovascular diseases and Alzheimer’s Disease. BioVision’s Endothelin Converting Enzyme 1 Activity Assay Kit utilizes the ability of active ECE-1 to cleave a synthetic substrate (MCA-based peptide) releasing free fluorophore, in the presence or absence of the ECE-1 Inhibitor Mix. The released f.luorophore can be easily quantified using a fluorescence microplate reader. Our kit uses a unique combination of substrate and inhibitor that specifically detects ECE-1 in a variety of biological samples. Contribution of other enzymes with similar catalytic properties - i.e. Endothelin Converting Enzyme 2 (ECE-2), Angiotensin-Converting Enzyme (ACE1, ACE2), and Neprilysin is compensated for within the assay. Our assay kit is simple, specific and can detect as low as 0.5 µU of ECE-1 activity.
  • Test
    Biovision supplies other types of Assays as 1.
  • Additional description
    Enzymes are cleaving the substrate. If the substrate is DNA they are called restriction enzymes. Activating enzymes will cut off the domain that is biological active to become functional.
  • Gene target
  • Short name
    Endothelin Converting Enzyme 1 Activity Assay Kit (Fluorometric)
  • Technique
    Enzyme, Assay, assays, enzymes
  • Host
    Assay
  • Alternative name
    Endothelin Converting Enzyme 1 Activity test reagent (fluorescence)
  • Alternative technique
    enzymes, arrays, kits
  • Alternative to gene target
    v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, transferase activity, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832
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