E.Coli Recombinant G6PD

• Catalog number
  6367-100
• Price
  Please ask
• Size
  100 ug

• Synonyms
  E.Coli Recombinant G6PD
• Alternative_names
  Glucose-6-phosphate 1-dehydrogenase, Zwf.
• Description
  Rate-limiting enzyme of the pentose phosphate pathway
• Recombinant
  Yes
• Source
  E. Coli
• Purity by SDS PAGE
  ≥90%
• Assay
  SDS-PAGE
• Biological activity
  Specific activity is 8-10 units/ml obtained by measuring the increase of NADPH in absorbance at 340 nm resulting from the reduction of NAD or NADP.
• Results
  8-10 units/ml
• Unit Definition
  One unit oxidizes 1.0 µmole D-glucose-6-phosphate to 6-phospho-D-gluconate per min in the presence of β-NADP at pH 7.4 at 25°C.
• Molecular Weight
  55.7 kDa (491 aa, 1-491 aa)
• Storage Temp
  -20°C
• Shipping
  gel pack
• Shelf Life
  12 months
• Concentration
  1 mg/ml
• Appearance
  Liquid
• Physical form description
  1 mg/ml solution in 50 mM MES pH 6.0, 0.1 mM PMSF, 2 mM EDTA, 0.5 mM DTT and 10% glycerol.
• Background Information
  Glucose-6-phosphate dehydrogenase is the rate-limiting enzyme of the pentose phosphate pathway, a metabolic pathway that supplies reducing energy to cells by maintaining the level of NADPH. G6PD converts glucose-6-phosphate into 6-phosphoglucono-delta-lactone and simultaneously produce NADPH. The NADPH in turn maintains the level of glutathione in these cells that helps protect the red blood cells against oxidative damage. G6PD deficiency cause acute hemolytic anemia.
• Amino acid sequence
  MAVTQTAQAC DLVIFGAKGD LARRKLLPSL YQLEKAGQLN PDTRIIGVGR ADWDKAAYTK VVREALETFM KETIDEGLWD TLSARLDFCN LDVNDTAAFS RLGAMLDQKN RITINYFAMP PSTFGAICKG LGEAKLNAKP ARVVMEKPLG TSLATSQEIN DQVGEYFEEC QVYRIDHYLG KETVLNLLAL RFANSLFVNN WDNRTIDHVE ITVAEEVGIE GRWGYFDKAG QMRDMIQNHL LQILCMIAMS PPSDLSADSI RDEKVKVLKS LRRIDRSNVR EKTVRGQYTA GFAQGKKVPG YLEEEGANKS SNTETFVAIR VDIDNWRWAG VPFYLRTGKR LPTKCSEVVV YFKTPELNLF KESWQDLPQN KLTIRLQPDE GVDIQVLNKV PGLDHKHNLQ ITKLDLSYSE TFNQTHLADA YERLLLETMR GIQALFVRRD EVEEAWKWVD SITEAWAMDN DAPKPYQAGT WGPVASVAMI TRDGRSWNEF E
• Handling
  Centrifuge the vial prior to opening.
• Usage
  For Research Use Only! Not to be used in humans
• Datasheet
• Additional description
  E. coli recombinant proteins are affinity purified and supplied lyophilized. For human research only.
• Additional source
  Recombinants or rec. proteins
• Group
  recombinants

• Gene target
  G6PD  
• Gene symbol
  G6PD
• Short name
  E Coli Recombinant G6PD
• Technique
  coli recombinant, Recombinant, from e. Coli, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. Biovision advises they will be reconstituted in a buffer soluion or culture medium for cell culture.
• Host
  Escherichia coli
• Alternative name
  Escherichia Coli Rec. glucose-6-phosphate dehydrogenase
• Alternative technique
  rec, escherichia
• Alternative to gene target
  glucose-6-phosphate dehydrogenase, G6PD1, G6PD and IDBG-91750 and ENSG00000160211 and 2539, NADP binding, nuclei, BT.65227 and IDBG-633035 and ENSBTAG00000019512 and 281179

Gene info

• Identity
  HGNC:4057
• Gene
  G6PD
• Long gene name
  glucose-6-phosphate dehydrogenase
• Synonyms
  • G6PD1
• GenBank acession
  • X03674
• Locus
  Xq28
• Discovery year
  2001-06-22
• Entrez gene record
  2539
• Pubmed identfication
  • 3012556
  • 2428611
• RefSeq identity
  • NM_000402
• VEGA ID
  OTTHUMG00000024237
• Locus Specific Databases
  • G6PD Mutations
  • Global Variome shared LOVD
  • LRG_148

MeSH Data

• Name
  Primary Cell Culture
• Concept
  Scope note: The initial culturing of cells derived directly from fresh TISSUES.
• Tree numbers
  • E01.370.225.500.223.500
  • E05.200.500.265.500
  • E05.242.223.500
  • E05.481.500.249.500
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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