E. Coli Recombinant SlyD

• Catalog number
  6366-100
• Price
  Please ask
• Size
  100 ug

• Synonyms
  E. Coli Recombinant SlyD
• Alternative_names
  FKBP-type peptidyl-prolyl cis-trans isomerase.
• Description
  Exhibits several activities including that of a peptidyl-prolyl isomerase
• Recombinant
  Yes
• Source
  E. Coli
• Purity by SDS PAGE
  ≥95%
• Assay
  SDS-PAGE
• Biological activity
  Specific activity is >220 nmoles/min/mg.
• Results
  >220 nmoles/min/mg
• Unit Definition
  One unit is defined as the amount of enzyme that cleaves 1 µmole of Suc-AAFP-pNA per minute at 25°C in Tris HCl pH 8.0 using chymotrypsin.
• Molecular Weight
  21 kDa (196 aa, 1-196 aa)
• Storage Temp
  -20°C
• Shipping
  gel pack
• Shelf Life
  12 months
• Concentration
  1 mg/ml
• Appearance
  Liquid
• Physical form description
  1 mg/ml solution in 20 mM Tris-HCl (pH 7.5).
• Background Information
  SlyD is a putative folding helper protein from the Escherichia coli cytosol, which consists of an N-terminal prolyl isomerase domain of the FKBP type and a presumably unstructured C-terminal tail. It is involved in the biosynthesis of the metal cluster in the [NiFe]-hydrogenase enzymes, and exhibits several activities including that of a peptidyl-prolyl isomerase.
• Amino acid sequence
  MKVAKDLVVS LAYQVRTEDG VLVDESPVSA PLDYLHGHGS LISGLETALE GHEVGDKFDV AVGANDAYGQ YDENLVQRVP KDVFMGVDEL QVGMRFLAET DQGPVPVEIT AVEDDHVVVD GNHMLAGQNL KFNVEVVAIR EATEEELAHG HVHGAHDHHH DHDHDGCCGG HGHDHGHEHG GEGCCGGKGN GGCGCH
• Handling
  Centrifuge the vial prior to opening.
• Usage
  For Research Use Only! Not to be used in humans
• Datasheet
• Additional description
  E. coli recombinant proteins are affinity purified and supplied lyophilized. For human research only.
• Additional source
  Recombinants or rec. proteins
• Group
  recombinants

• Gene target
  SlyD  
• Short name
  Recombinant SlyD
• Technique
  coli recombinant, Recombinant, from e. Coli, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. Biovision advises they will be reconstituted in a buffer soluion or culture medium for cell culture.
• Host
  e. coli, Escherichia coli, Recombination of bioactive proteins and longer peptides in Escherichia Coli is done often with His tagging. Biovision supplies Rec. E. Coli affinity purified or tag purified antigens in 1 quantities or bulk volumes on request.
• Species
  E. coli, E. coli
• Alternative name
  E. Coli Rec. SlyD
• Alternative technique
  rec, escherichia

MeSH Data

• Name
  Primary Cell Culture
• Concept
  Scope note: The initial culturing of cells derived directly from fresh TISSUES.
• Tree numbers
  • E01.370.225.500.223.500
  • E05.200.500.265.500
  • E05.242.223.500
  • E05.481.500.249.500
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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