E. Coli Recombinant SlyD
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Catalog number6366-100
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PricePlease ask
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Size100 ug
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SynonymsE. Coli Recombinant SlyD
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Alternative_namesFKBP-type peptidyl-prolyl cis-trans isomerase.
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DescriptionExhibits several activities including that of a peptidyl-prolyl isomerase
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RecombinantYes
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SourceE. Coli
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Purity by SDS PAGE≥95%
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AssaySDS-PAGE
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Biological activitySpecific activity is >220 nmoles/min/mg.
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Results>220 nmoles/min/mg
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Unit DefinitionOne unit is defined as the amount of enzyme that cleaves 1 µmole of Suc-AAFP-pNA per minute at 25°C in Tris HCl pH 8.0 using chymotrypsin.
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Molecular Weight21 kDa (196 aa, 1-196 aa)
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Storage Temp-20°C
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Shippinggel pack
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Shelf Life12 months
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Concentration1 mg/ml
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AppearanceLiquid
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Physical form description1 mg/ml solution in 20 mM Tris-HCl (pH 7.5).
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Background InformationSlyD is a putative folding helper protein from the Escherichia coli cytosol, which consists of an N-terminal prolyl isomerase domain of the FKBP type and a presumably unstructured C-terminal tail. It is involved in the biosynthesis of the metal cluster in the [NiFe]-hydrogenase enzymes, and exhibits several activities including that of a peptidyl-prolyl isomerase.
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Amino acid sequenceMKVAKDLVVS LAYQVRTEDG VLVDESPVSA PLDYLHGHGS LISGLETALE GHEVGDKFDV AVGANDAYGQ YDENLVQRVP KDVFMGVDEL QVGMRFLAET DQGPVPVEIT AVEDDHVVVD GNHMLAGQNL KFNVEVVAIR EATEEELAHG HVHGAHDHHH DHDHDGCCGG HGHDHGHEHG GEGCCGGKGN GGCGCH
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HandlingCentrifuge the vial prior to opening.
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UsageFor Research Use Only! Not to be used in humans
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Additional descriptionE. coli recombinant proteins are affinity purified and supplied lyophilized. For human research only.
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Additional sourceRecombinants or rec. proteins
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Grouprecombinants
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Gene target
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Short nameRecombinant SlyD
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Techniquecoli recombinant, Recombinant, from e. Coli, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. Biovision advises they will be reconstituted in a buffer soluion or culture medium for cell culture.
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Hoste. coli, Escherichia coli, Recombination of bioactive proteins and longer peptides in Escherichia Coli is done often with His tagging. Biovision supplies Rec. E. Coli affinity purified or tag purified antigens in 1 quantities or bulk volumes on request.
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SpeciesE. coli, E. coli
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Alternative nameE. Coli Rec. SlyD
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Alternative techniquerec, escherichia
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MeSH Data
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Name
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ConceptScope note: The initial culturing of cells derived directly from fresh TISSUES.
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Tree numbers
- E01.370.225.500.223.500
- E05.200.500.265.500
- E05.242.223.500
- E05.481.500.249.500
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data