E. coli Recombinant IDNK

• Catalog number
  P1050-10
• Price
  Please ask
• Size
  10 ug

• Synonyms
  ECK4261, gntV, JW4225
• Alternative_names
  ECK4261, gntV, JW4225
• Description
  Protein involved in the pathway L-idonate degradation, which is part of Carbohydrate acid metabolism.
• Recombinant
  Yes
• Source
  E. coli
• Purity by SDS PAGE
  > 90%
• Assay
  SDS-PAGE
• Activity Specifications test method
  Measured by its ability to convert 1.0 umole of D-gluconate to 6-phospho-D-gluconate per minute at pH 8.0 at 37C.
• Biological activity
  Specific activity is > 80 units/mg, in which one unit will convert 1.0 umole of D-gluconate to 6-phospho-D-gluconate per minute at pH 8.0 at 37C.
• Results
  > 80 units/mg
• Unit Definition
  One unit is the amount of enzyme that will convert 1.0 umole of D-gluconate to 6-phospho-D-gluconate per minute at pH 8.0 at 37C.
• Molecular Weight
  23.4 kDa
• Storage Temp
  -20°C
• Shipping
  Gel Pack
• Shelf Life
  12 months
• Appearance
  Liquid
• Physical form description
  In 20mM Tris-HCl buffer (pH 8.0) containing 0.15M NaCl, 10% glycerol, 1mM DTT
• Background Information
  D-gluconate kinase, thermosensitive, also known as idnk, D-gluconate kinase, thermosensitive, also known as idnk, is a 187 amino acid protein that belongs to the gluconokinase gntK/gntV family and catalyzes the conversion of ATP and D-gluconate to ADP and 6-phospho-D-gluconate. This protein is thought to play a role in gender determination, deletion of the distal portion of 9p can lead to development of male to female sex reversal, the phenotype of a female with a male X, Y genotype. Recombinant E.coli idnk protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.
• Amino acid sequence
  MGSSHHHHHH SSGLVPRGSH MGSMAGESFI LMGVSGSGKT LIGSKVAALL SAKFIDGDDL HPAKNIDKMS QGIPLSDEDR LPWLERLNDA SYSLYKKNET GFIVCSSLKK QYRDILRKGS PHVHFLWLDG DYETILARMQ RRAGHFMPVA LLKSQFEALE RPQADEQDIV RIDINHDIAN VTEQCRQAVL AIRQNRICAK EGSASDQRCE
• Handling
  Centrifuge the vial prior to opening.
• Usage
  For Research Use Only! Not to be used in humans
• Datasheet
• Additional description
  E. coli recombinant proteins are affinity purified and supplied lyophilized. For human research only.
• Additional source
  Recombinants or rec. proteins
• Group
  recombinants

• Gene target
  IDNK  
• Gene symbol
  IDNK
• Short name
  Recombinant IDNK
• Technique
  coli recombinant, Recombinant, from e. Coli, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. Biovision advises they will be reconstituted in a buffer soluion or culture medium for cell culture.
• Host
  e. coli, Escherichia coli, Recombination of bioactive proteins and longer peptides in Escherichia Coli is done often with His tagging. Biovision supplies Rec. E. Coli affinity purified or tag purified antigens in 1 quantities or bulk volumes on request.
• Species
  E. coli, E. coli
• Alternative name
  E. coli Rec. IDNK
• Alternative technique
  rec, escherichia

Gene info

• Identity
  HGNC:31367
• Gene
  IDNK
• Long gene name
  IDNK gluconokinase
• Synonyms gene
  • C9orf103
• Synonyms gene name
  • chromosome 9 open reading frame 103
  • idnK, gluconokinase homolog (E. coli)
  • IDNK, gluconokinase
• Synonyms
  • bA522I20.2
  • hGntK
• GenBank acession
  • BC036421
• Locus
  9q21.32
• Discovery year
  2004-06-02
• Entrez gene record
  414328
• Pubmed identfication
  • 23067238
  • 24896608
  • 26505675
• RefSeq identity
  • NM_001001551
• VEGA ID
  OTTHUMG00000020105

MeSH Data

• Name
  Primary Cell Culture
• Concept
  Scope note: The initial culturing of cells derived directly from fresh TISSUES.
• Tree numbers
  • E01.370.225.500.223.500
  • E05.200.500.265.500
  • E05.242.223.500
  • E05.481.500.249.500
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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