E. coli Recombinant Carbonic anhydrase

• Catalog number
  P1049-50
• Price
  Please ask
• Size
  50 ug

• Synonyms
  Carbonate dehydratase, CAN, yadF
• Alternative_names
  Carbonate dehydratase, CAN, yadF
• Description
  Catalyzes the reversible hydration of carbon dioxide in various biological processes.
• Recombinant
  Yes
• Source
  E. coli
• Purity by SDS PAGE
  > 95%
• Assay
  SDS-PAGE
• Activity Specifications test method
  Measured by its ability to hydrolyze 1.0 pmole of 4-nitrophenyl acetate to 4-nitrophenol per minute at pH 7.5 at 37C.
• Biological activity
  Specific activity is defined as the amount of enzyme that hydrolyze 1.0 pmole of 4-nitrophenyl acetate to 4-nitrophenol per minute at pH 7.5 at 37C.
• Results
  >1,000 pmol/min/ug
• Molecular Weight
  27 kDa
• Storage Temp
  -20°C
• Shipping
  Gel Pack
• Shelf Life
  12 months
• Appearance
  Liquid
• Physical form description
  In 20 mM Tris-HCl buffer (pH8.0) containing 1mM DTT, 10% glycerol
• Background Information
  Carbonic anhydrase (CA) is an enzyme that catalyses rapid conversion of carbon dioxide to bicarbonate and protons (CO2 + H2O ↔ HCO3− + H+). Most carbonic anhydrases contain a zinc ion in their active site and the primary function of this enzyme is known to maintain acid-base balance in blood and other tissues, and to help transport carbon dioxide of tissues. Carbonic anhydrases have been found in all kingdoms of life. Recombinant carbonic anhydrase fused to His-tag, was expressed in E.coli and purified by conventional chromatography techniques.
• Amino acid sequence
  MGSSHHHHHH SSGLVPRGSH MKDIDTLISN NALWSKMLVE EDPGFFEKLA QAQKPRFLWI GCSDSRVPAE RLTGLEPGEL FVHRNVANLV IHTDLNCLSV VQYAVDVLEV EHIIICGHYG CGGVQAAVEN PELGLINNWL LHIRDIWFKH SSLLGEMPQE RRLDTLCELN VMEQVYNLGH STIMQSAWKR GQKVTIHGWA YGIHDGLLRD LDVTATNRET LEQRYRHGIS NLKLKHANHK
• Handling
  Centrifuge the vial prior to opening.
• Usage
  For Research Use Only! Not to be used in humans
• Datasheet
• Additional description
  E. coli recombinant proteins are affinity purified and supplied lyophilized. For human research only.
• Additional source
  Recombinants or rec. proteins
• Group
  recombinants

• Gene target
  Carbonic   anhydrase  
• Short name
  Recombinant Carbonic anhydrase
• Technique
  coli recombinant, Recombinant, from e. Coli, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. Biovision advises they will be reconstituted in a buffer soluion or culture medium for cell culture.
• Host
  e. coli, Escherichia coli, Recombination of bioactive proteins and longer peptides in Escherichia Coli is done often with His tagging. Biovision supplies Rec. E. Coli affinity purified or tag purified antigens in 1 quantities or bulk volumes on request.
• Species
  E. coli, E. coli
• Alternative name
  E. coli Rec. Carbonic anhydrase
• Alternative technique
  rec, escherichia

MeSH Data

• Name
  Primary Cell Culture
• Concept
  Scope note: The initial culturing of cells derived directly from fresh TISSUES.
• Tree numbers
  • E01.370.225.500.223.500
  • E05.200.500.265.500
  • E05.242.223.500
  • E05.481.500.249.500
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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