DNA Library Prep Kit for IIlumina Sequencing
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Catalog number
K1475-12
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Price
Please ask
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Size
12 Rxns
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Description
To prepare DNA samples for Illumina Sequencing
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Summary
DNA library is prepared for Illumina sequencing by end repair, adenylation, ligation with adapter sequences and amplification
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Detection Method
DNA library is prepared for Illumina Sequencing by end repair, adenylation, ligation with adapter sequences and PCR amplification
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Applications
Library preparation for Whole Genome Sequencing, ChIP-Seq, Amplicon Sequencing and cDNA Sequencing
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Sample Type
Purified fragmented DNA (recommended size range of 200-500 bp) or PCR amplicons
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Features Benefits
Wide range of input amounts (2-500 ng) and sample types, including amplicons and fragmented DNA,High quality Illumina compatible libraries,Minimal hands on time and short 2 hour library preparation workflow
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Storage Conditions
-20°C
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Shipping Conditions
Gel Pack
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Shelf life
12 months
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Background
BioVision’s DNA Library Prep Kit for IIlumina Sequencing is used to efficiently convert (2 ng-500 ng) of DNA fragments into fully compatible libraries for Illumina Next Generation Sequencing (NGS) platforms. The sample preparation step involves the preparation of a DNA library by fragmentation, end repair, adenylation, ligation with adapter sequences and amplification. The DNA library is then loaded onto the chips for amplification and sequencing. The presence of adapter sequences allows the hybridization of the libraries to the sequencing chips and provides a universal priming site for sequencing primers. BioVision’s DNA Library Prep Kit contains index adapters needed for running multiple samples on a single Illumina flow cell as well as the magnetic beads for purification, which is required for the reaction cleanup after ligation and PCR steps. The entire procedure can be completed within 2 hours.
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Test
Sequencing primers for deep sequencing kits give sequencing nucleic acid results of DNA libraries, cDNAs. The MiSeq, HiSeq and NextSeq sequencing primers are designed to sequence the whole transcriptome.
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Gene target
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Gene symbol
PRKDC, HLA-DOA
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Short name
DNA Library Prep Kit for IIlumina Sequencing
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Technique
sequencing, dna, sequencings
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Alternative name
Desoxyribonucleic acid collection preparation reagent reagent to measure IIlumina Sequencing
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Alternative technique
sequencings, kits
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Alternative to gene target
v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, transferase activity, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832
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Gene info
Gene info
MeSH Data
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Name
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Concept
Scope note:
A multistage process that includes cloning, physical mapping, subcloning, determination of the DNA SEQUENCE, and information analysis.
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Tree numbers
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Qualifiers
ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
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