Deproteinizing Sample Preparation Kit II
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Catalog number
K823-200
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Price
Please ask
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Size
200 assays
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Description
Non-PCA method to deproteinize samples
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Summary
Unique tool to prepare samples for the analysis of a variety of small molecules. Proteins are precipitated without using perchloric acid (PCA)
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Sample Type
• Biofluids: serum, plasma, whole blood, saliva, urine etc. • Lysates: cell culture, mammalian tissue, yeast, bacteria etc.
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Features Benefits
• Fast and convenient • Samples prepared using this kit can be directly used in a wide variety of small molecule bioassays.
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Storage Conditions
RT
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Shipping Conditions
RT
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Shelf life
12 months
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Background
The study of biofluids, cell, bacteria, yeast, and tissue lysates has delivered vital information regarding their biochemical composition. These biological samples comprise a rich, complex list of lipids, proteins, small organic molecules, ions, and, depending on the sample, exogenous molecules (i.e. drugs). Several small organic molecules can also be found free and/or bound to other macromolecules. These macromolecules (lipoproteins, proteins, enzymes) interfere or degrade low molecular-weight metabolites. Therefore, sample deproteinization is often required. Chemical deproteinization procedure such as Trichloroacetic acid (TCA) is one of the most utilized methods to accomplish sample deproteinization. BioVision’s Deproteinizing Sample Preparation Kit II offers an excellent alternative when metabolite lability affects the accurate detection and estimation of small molecule concentrations and when organic solvents or ultrafiltration cannot be used. TCA precipitates proteins by lowering the sample pH drastically. After removal of precipitated proteins, the pH of the sample is neutralized with neutralization buffer that is provided in the kit. The kit is easy to follow, convenient, and can be utilized for the preparation of a large number of samples in parallel. Samples prepared using this kit can be directly used in various bioassays.
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Gene target
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Short name
Deproteinizing Sample Preparation Kit II
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Technique
Sample, samples
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Alternative name
Deproteinizing Sample Preparation reagent II
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Alternative technique
samples, kits
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Alternative to gene target
v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, transferase activity, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832
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Product images
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