Deproteinizing Sample Preparation Kit II

  • Catalog number
    K823-200
  • Price
    Please ask
  • Size
    200 assays
  • Description
    Non-PCA method to deproteinize samples
  • Summary
    Unique tool to prepare samples for the analysis of a variety of small molecules. Proteins are precipitated without using perchloric acid (PCA)
  • Sample Type
    • Biofluids: serum, plasma, whole blood, saliva, urine etc. • Lysates: cell culture, mammalian tissue, yeast, bacteria etc.
  • Features Benefits
    • Fast and convenient • Samples prepared using this kit can be directly used in a wide variety of small molecule bioassays.
  • Storage Conditions
    RT
  • Shipping Conditions
    RT
  • Shelf life
    12 months
  • Background
    The study of biofluids, cell, bacteria, yeast, and tissue lysates has delivered vital information regarding their biochemical composition. These biological samples comprise a rich, complex list of lipids, proteins, small organic molecules, ions, and, depending on the sample, exogenous molecules (i.e. drugs). Several small organic molecules can also be found free and/or bound to other macromolecules. These macromolecules (lipoproteins, proteins, enzymes) interfere or degrade low molecular-weight metabolites. Therefore, sample deproteinization is often required. Chemical deproteinization procedure such as Trichloroacetic acid (TCA) is one of the most utilized methods to accomplish sample deproteinization. BioVision’s Deproteinizing Sample Preparation Kit II offers an excellent alternative when metabolite lability affects the accurate detection and estimation of small molecule concentrations and when organic solvents or ultrafiltration cannot be used. TCA precipitates proteins by lowering the sample pH drastically. After removal of precipitated proteins, the pH of the sample is neutralized with neutralization buffer that is provided in the kit. The kit is easy to follow, convenient, and can be utilized for the preparation of a large number of samples in parallel. Samples prepared using this kit can be directly used in various bioassays.
  • Gene target
  • Short name
    Deproteinizing Sample Preparation Kit II
  • Technique
    Sample, samples
  • Alternative name
    Deproteinizing Sample Preparation reagent II
  • Alternative technique
    samples, kits
  • Alternative to gene target
    v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, transferase activity, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832
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