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Alternative_name
2-(4-Amidinophenyl)-6-indolecarbamidine dihydrochloride; 4′,6-Diamidino-2-phenylindole dihydrochloride
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Description
DAPI is a fluorescent, cell-permeable DNA-binding dye preferentially binds to DNA rich in adenine and thymine. DAPI is useful in measuring nuclear DNA content using flow cytometry for sorting isolated chromosomes and is also useful for microscopic detection of nuclei and nuclear DNA in normal and apoptotic cells. DAPI can also be used to detect mycoplasma and in simple retrograde double-labelling procedures for studying axonal branching employing Evans Blue and Primulin.
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CAS Number
28718-90-3
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Structure Available
Yes
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Salt Form
Yes
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Molecular Formula
C₁₆H₁₅N₅.2HCl
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Molecular Weight
350.25
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Cell Permeable
Yes
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Purity
≥98% by HPLC
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Solubilities
DMSO (>35 mg/ml)
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Handling
Protect from air and light
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Tag Line
A useful nuclear and chromosome counterstain
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Storage Condition
-20°C
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Shipping Condition
gel pack
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Shelf Life
36 months
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MDL Number
NL5995050
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PubChem CID
160166
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SMILES
C1=CC(=CC=C1C2=CC3=C(N2)C=C(C=C3)C(=N)N)C(=N)N.Cl.Cl
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InChi
InChI=1S/C16H15N5.2ClH/c17-15(18)10-3-1-9(2-4-10)13-7-11-5-6-12(16(19)20)8-14(11)21-13;;/h1-8,21H,(H3,17,18)(H3,19,20);2*1H
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InChi Key
FPNZBYLXNYPRLR-UHFFFAOYSA-N
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MSDS Available
Yes