Cytochrome c Releasing Apoptosis Assay Kit

• Catalog number
  K257-100
• Price
  Please ask
• Size
  100 assays

• Summary
  • Detection method- Western blotting • Species reactivity- Mammalian • Applications- Effective means for detecting cytochrome c translocation from mitochondria into cytosol during apoptosis. The kit provides an easy-to-use procedure and reagents for separating a mitochondria-enriched fraction from cytosol.
• Sample Type
  Cells and tissues
• Features Benefits
  • Simple procedure; takes ~3 hours • Fast and convenient • Cytochrome c releasing from mitochondria into cytosol is then determined by Western blotting using the cytochrome c antibody provided in the kit. The procedure is simple and straightforward. No ultracentrifugation is required.
• Storage Conditions
  -20°C
• Shipping Conditions
  gel pack
• Shelf life
  12 months
• Background
  Cytochrome c plays an important role in apoptosis. The protein is located in the space between the inner and outer mitochondrial membranes. An apoptotic stimulus triggers the release of cytochrome c from the mitochondria into cytosol where it binds to Apaf-1. The cytochrome c/Apaf-1 complex activates caspase-9, which then activates caspase-3 and other downstream caspases. BioVision’s Cytochrome c Releasing Apoptosis Assay Kit provides an effective means for detecting cytochrome c translocation from mitochondria into cytosol during apoptosis. The kit provides unique formulations of reagents to isolate a highly enriched mitochondria fraction from cytosol. The procedure is so simple and easy to perform, no ultracentrifugation is required and no toxic chemicals are involved. Cytochrome c releasing from mitochondria into cytosol is then determined by Western blotting using the cytochrome c antibody provided in the kit.
• Data Sheet Link
• Additional description
  This 1 is suited for programmed cell-death studies.
• Test
  Biovision supplies other types of Assays as 1.

• Gene target
  Cytochrome   Releasing   Kit  
• Short name
  Cytochrome c Releasing Apoptosis Assay Kit
• Technique
  apoptosis, Assay, apoptosis pathway, assays
• Host
  Assay
• Alternative name
  Cytochrome c Releasing programmed cellular destruction test reagent
• Alternative technique
  celldeath, arrays, kits
• Alternative to gene target
  v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, transferase activity, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832

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