Creatine Kinase (CK) Activity Colorimetric Assay Kit
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Catalog number
K777-100
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Price
Please ask
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Size
100 assays
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Description
Sensitive Assay, HTS
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Summary
• Detection method- Absorbance (450 nm) • Application- The kit can detect creatine kinase activity less than 1 mU.
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Sample Type
• Serum & plasma. • Animal tissues: muscle, brain, heart etc. • Cell culture: Adherent or suspension cells.
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Features Benefits
• Simple procedure; takes ~ 30-40 minutes • Sensitive and high-throughput adaptable • Kit contains all necessary reagents for measuring creatine kinase activity
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Storage Conditions
-20°C
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Shipping Conditions
gel pack
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Shelf life
12 months
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Background
Creatine Kinase (CK) also known as creatine phosphokinase (CPK) and ATP: creatine N-phosphotransferase is a common cellular enzyme (EC 2.7.3.2). It catalyzes the reversible conversion of creatine and ATP into ADP and phosphocreatine. CK is widely expressed in various tissues and cell types, with highest activity in striated muscles, heart tissue and brain. CK consists of two subunits: M (muscle) and B (brain), and has three isoenzymes: CK-MM (skeleton muscle), CK-MB (cardiac muscle), and CK-BB (brain). Increased CK level is associated with many diseases such as myocardial infarction, muscular dystrophy, pulmonary infarction and brain tumors. Accurate measurement of CK is crucial for early diagnosis, prediction and therapeutic strategy. In BioVision’s Creatine Kinase Activity Colorimetric Assay kit, creatine kinase converts creatine into phosphocreatine and ADP. The generated phoshocreatine and ADP reacts with CK Enzyme Mix to form an intermediate, which reduces a colorless Probe to a colored product with strong absorbance at 450 nm. The CK Activity Assay is high-throughput adaptable, simple and sensitive. This assay kit can detect Creatine Kinase activity less than 1 mU.
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Test
Biovision supplies other types of Assays as 1.
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Properties
Colorimetric assays or detection use UV absorption or enzymatic color reaction.
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Gene target
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Short name
Creatine Kinase (CK) Activity Colorimetric Assay Kit
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Technique
colorimetric, Assay, assays, colorimetry
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Host
Assay
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Alternative name
Creatine phosphorylation catalyst (CK) Activity colorimetry test reagent
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Alternative technique
assays, arrays, kits
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Alternative to gene target
v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, transferase activity, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832
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