Collagenase (Collagen Degradation/Zymography) Assay Kit (Fluorometric)

  • Catalog number
    K490-100
  • Price
    Please ask
  • Size
    100 assays
  • Description
    Sensitive Fluorometric Assay to Detect Collagenase in Mammalian Samples
  • Summary
    • Detection method- Fluorometric (490/520 nm) • Species reactivity- mammalian • Applications- - Measurement of collagenase activity - For screening/studying/characterizing collagenase inhibitors
  • Detection Method
    Fluorescence (490/520 nm)
  • Species Reactivity
    Mammalian
  • Applications
    • Measurement of collagenase activity • For screening/studying/characterizing collagenase inhibitors
  • Sample Type
    Purified Collagenase, cell lysates, tissues lysates
  • Features Benefits
    • Rapid & sensitive • Simple assay to measure collagenase activity as well as to screen/study/characterize potential inhibitors of collagenase • Includes Fluorometric Collagenase Substrate and Positive Control
  • Storage Conditions
    -20°C
  • Shipping Conditions
    gel pack
  • Shelf life
    12 months
  • Background
    Collagenases, members of the matrix metalloproteinase (MMP) family, are endopeptidases that digest native collagen in the triple helix region and are involved in the physiological and pathological turnover of connective tissues. Collagens are the major fibrous component of animal extracellular connective tissue. Bacterial collagenases differ from vertebrate collagenases in that they exhibit broader substrate specificity. Collagenases may play a role in facilitating tumor cell invasion of the extracellular matrix at multiple stages of the metastatic process. Collagenases have also been approved for medical uses for the treatment of Dupuytren's contracture, Peyronie's disease and wound healing. Collagenase activity is usually detected by small peptide-based activity assays which may suffer from lack of substrate specificity. Other methods for collagenase activity include collagen Zymography where samples are electrophoresed on a collagen-containing SDS-PAGE, and further renatured in a suitable buffer for 12-16 h. The zymogram is subsequently stained, and areas of digestion appear as clear bands against a darkly stained background where the substrate has been degraded by the enzyme. Such methods are laborious, time-consuming and may lead to the loss of enzymatic activity as renaturation may not be completely reversible. BioVision’s Collagenase Activity Assay Kit utilizes a hybrid approach for the detection of collagenase activity by employing a highly quenched collagen substrate which upon cleavage by a suitable collagenase releases a fluorophore, which can be easily quantified using a conventional microplate reader. This method is substrate-specific, simple, fast, high-throughput adaptable and amenable to the sensitive detection of collagenase activity (as low as 0.6 mCDU for bacterial collagenase) in biological samples
  • Test
    Biovision supplies other types of Assays as 1.
  • Gene
    Collagen Types in bones and muscles can be hydrolyzed and used in human grafts. ( Type II )
  • Gene target
  • Short name
    Collagenase (Collagen Degradation/Zymography) Assay Kit (Fluorometric)
  • Technique
    Assay, assays
  • Host
    Assay
  • Alternative name
    Collagenase (Collagen Degradation/Zymography) test reagent (fluorescence)
  • Alternative technique
    arrays, kits
  • Alternative to gene target
    v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, transferase activity, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832
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