CNTF, rat recombinant
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Catalog number4019-1000
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Price:
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Size1 mg
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SynonymsHCNTF, CNTF, Ciliary Neurotrophic Factor.
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Alternative_namesHCNTF, CNTF, Ciliary Neurotrophic Factor.
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DescriptionA potent neural factor promoting survivability and differentiation of neuronal cell types.
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RecombinantYes
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SourceE. coli
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Purity by SDS PAGE≥99%
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AssaySDS-PAGE
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Biological activityFully biologically active by its ability to phosphorylate STAT3 in several cells lines.
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Molecular Weight22.834 kDa
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Storage Temp-20°C
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ShippingGel pack
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Shelf Life12 months
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AppearanceLyophilized protein
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Physical form descriptionLyophilized from 0.025 % NaHCO₃
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Reconstitution InstructionsCentrifuge the vial prior to opening. Reconstitute in sterile ddH₂O or 0.4 % NaHCO₃ adjusted to pH 8-9 to a concentration ≥ 100 µg/ml. This solution can then be diluted into other aqueous buffer, preferably in the presence of carrier protein.
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Background InformationCNTF is a polypeptide hormone whose actions appear to be restricted to the nervous system where it promotes neurotransmitter synthesis and neurite outgrowth in certain neuronal populations. The protein is a potent survival factor for neurons and oligodendrocytes and may be relevant in reducing tissue destruction during inflammatory attacks. A mutation in this gene, which results in aberrant splicing, leads to ciliary neurotrophic factor deficiency, but this phenotype is not causally related to neurologic disease. CNTF is a survival factor for various neuronal cell types and seems to prevent the degeneration of motor axons after axotomy. CNTF Recombinant Rat produced in E.Coli is a single, non-glycosylated polypeptide chain containing 200 amino acids and having a molecular mass of 22834 Dalton. The CNTF is purified by proprietary chromatographic techniques.
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Amino acid sequenceAFAEQTPLTLHRRDLSSRSIWLARKIRSDLTALMESYVKHQGLNKNINLDSVDGVPVASTDRWSEMTEAERLQENLQAYRTFQGMLTKLLEDQRVHFTPTEGDFHQAIHTLMLQVSAFAYQLEELMVLLEQKIPENEADGMPATVGDGGLFEKKLWGLKVLQELSQWTVRSIHDLRVISSHQMGISALESHYGAKDKQM
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HandlingCentrifuge the vial prior to opening.
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UsageFor Research Use Only! Not to be used in humans
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AboutRats are used to make rat monoclonal anti mouse antibodies. There are less rat- than mouse clones however. Rats genes from rodents of the genus Rattus norvegicus are often studied in vivo as a model of human genes in Sprague-Dawley or Wistar rats.
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Latin nameRattus norvegicus
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Additional sourceRecombinants or rec. proteins
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Grouprecombinants
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Gene target
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Gene symbolZFP91-CNTF, CNTF
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Short nameCNTF, recombinant
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TechniqueRecombinant, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. Biovision advises they will be reconstituted in a buffer soluion or culture medium for cell culture.
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HostRat
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SpeciesRat, Rats
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Alternative nameciliary neurotrophic factor, rat Rec.
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Alternative techniquerec
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Alternative to gene targetciliary neurotrophic factor, HCNTF, CNTF and IDBG-409278 and ENSG00000242689 and 1270, growth factor activity, Extracellular, Cntf and IDBG-254935 and ENSMUSG00000079415 and 12803, CNTF and IDBG-637978 and ENSBTAG00000003624 and 100848228
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Gene info
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Identity
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Gene
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Long gene nameZFP91-CNTF readthrough (NMD candidate)
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GenBank acession
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Locus
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Discovery year2011-05-31
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameciliary neurotrophic factor
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year1991-01-07
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Interleukin 6 type cytokine family
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VEGA ID
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Locus Specific Databases
MeSH Data
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Name
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ConceptScope note: The initial culturing of cells derived directly from fresh TISSUES.
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Tree numbers
- E01.370.225.500.223.500
- E05.200.500.265.500
- E05.242.223.500
- E05.481.500.249.500
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data