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Alternative_name
2′-(4-Ethoxyphenyl)-5-(4-methyl-1-piperazinyl)-2,5′-bi-1H-benzimidazole trihydrochloride, HOE 33342, Hoechst 33342, bisBenzimide
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Description
A cell-permeable fluorescent DNA stain with low cytotoxicity that binds preferentially to adenine-thymine (A-T) regions of DNA. This stain binds into the minor groove of DNA and exhibits distinct fluorescence emission spectra that are dependent on dye:base pair ratios. May be used for fluorescence microscopy or flow cytometry.
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CAS Number
23491-52-3
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Structure Available
Yes
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Salt Form
No
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Molecular Formula
C₂₇H₂₈N₆O · 3HCl
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Molecular Weight
561.93
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Cell Permeable
Yes
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Purity
≥98% by HPLC
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Solubilities
H₂O (~ 50 mg/ml)
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Handling
Protect from light and moisture
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Tag Line
A cell-permeable fluorescent DNA stain
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Storage Condition
-20°C
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Shipping Condition
gel pack
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Shelf Life
24months
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MDL Number
MFCD00012678
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PubChem CID
16760503
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SMILES
CCOC1=CC=C(C=C1)C2=NC3=C(N2)C=C(C=C3)C4=NC5=C(N4)C=C(C=C5)N6CCN(CC6)C.Cl.Cl.Cl
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InChi
InChI=1S/C27H28N6O.3ClH/c1-3-34-21-8-4-18(5-9-21)26-28-22-10-6-19(16-24(22)30-26)27-29-23-11-7-20(17-25(23)31-27)33-14-12-32(2)13-15-33;;;/h4-11,16-17H,3,12-15H2,1-2H3,(H,28,30)(H,29,31);3*1H
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InChi Key
JABNPSKWVNCGMX-UHFFFAOYSA-N
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MSDS Available
Yes
