Alternative_name
2′-(4-Hydroxyphenyl)-5-(4-methyl-1-piperazinyl)-2,5′-bi(1H-benzimidazole) trihydrochloride, 2-[2-(4-Hydroxyphenyl)-6-benzimidazoyl]-6-(1-methyl-4-piperazyl)benzimidazole trihydrochloride, BBIH, HOE 33258, Hoechst 33258
Description
A fluorescent DNA stain with low cytotoxicity that binds preferentially to adenine-thymine (A-T) regions of DNA. Useful for staining DNA, chromosomes and nuclei. May be used for fluorescence microscopy or flow cytometry.
CAS Number
23491-45-4
Structure Available
Yes
Salt Form
No
Molecular Formula
C₂₅H₂₄N₆O · 3HCl
Molecular Weight
533.88
Cell Permeable
Yes
Purity
≥98% by HPLC
Solubilities
H₂O (~ 10 mg/ml)
Handling
Protect from light and moisture
Tag Line
A fluorescent DNA stain
Storage Condition
-20°C
Shipping Condition
gel pack
Shelf Life
24months
MDL Number
MFCD00012679
PubChem CID
5360743
SMILES
CN1CCN(CC1)C2=CC3=C(C=C2)N=C(N3)C4=CC5=C(C=C4)NC(=C6C=CC(=O)C=C6)N5.Cl.Cl.Cl
InChi
InChI=1S/C25H24N6O.3ClH/c1-30-10-12-31(13-11-30)18-5-9-21-23(15-18)29-25(27-21)17-4-8-20-22(14-17)28-24(26-20)16-2-6-19(32)7-3-16;;;/h2-9,14-15,26,28H,10-13H2,1H3,(H,27,29);3*1H
InChi Key
OKQAASMPIOSOEX-UHFFFAOYSA-N
MSDS Available
Yes
