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Antibody Target
ATGL
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Target Alternative Name
Adipose Triglyceride Lipase, Patatin-like phospholipase domain-containing protein 2, Desnutrin, Transport-secretion protein 2, TTS2
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Antibody Type
Polyclonal
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Species Reactivities
Human, mouse, rat
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Immunogen Sequence
Synthetic peptide corresponding to residues surrounding amino acids 385 of human ATGL
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Accession
AAW81962
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Gene Id
57104
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Appearance
Colorless liquid
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Form
Liquid
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Concentration
0.5 mg/ml
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Formulation
100 µg (0.5 mg/ml) affinity purified rabbit polyclonal antibody in phosphate-buffered saline (PBS) containing 30% glycerol, 0.5% BSA, and 0.01% thimerosal.
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Purification
Affinity purified
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Application
Western blot, Immunoprecipitation, and Immunohistochemistry
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Positive Control
Western Blot: rat kidney tissue lysate. IHC: Heart tissue
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Application And Usages
Western blotting (0.5-4 µg/ml), Immunohistochemistry (5 µg/ml), and immunoprecipitation. recognizes 54 kDa ATGL in samples from human, mouse and rat origins. However, the optimal concentrations should be determined individually.
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Description
Triglycerides form an important energy store in many living organisms. Adipose tissue serves as the primary storage depot for triglycerides in mammals. Lipolytic enzymes mobilize triglycerides during periods of starvation to provide organisms with necessary energy. Hormone-sensitive lipase (HSL), the first identified lipolytic enzyme, hydrolyzes triglycerides in mammalian adipose tissues. Additional lipolytic enzymes, including adipose triglyceride lipase (ATGL), have also been discovered. The primary function of ATGL is to catalyze the hydrolysis of the first ester bond of lipid molecules. This enzyme may provide diglyceride substrates for HSL hydrolysis. ATGL is abundantly expressed in murine white and brown adipose tissue, and is highly substrate specific. ATGL was independently identified as desnutrin and the TG-hydrolace inducible phospholipase-A2-z.
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Storage Condition
-20 °C
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Shipping Condition
gel pack
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Shelf Life
12 months
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Usage
For Research Use Only! Not to be used in humans.
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Handling
The antibody solution should be gently mixed before use.
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Western Blot Verified
Verified
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Immunocytochemistry Verified
Verified
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Immunofluorescence Verified
Not Verified
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Immunoprecipitation Verified
Not Verified
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FACS Verified
Not Verified
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ELISA Verified
Not Verified
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ChIP Verified
Not Verified
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Flow Cytometry Verified
Not Verified
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