Anti-CD54 / ICAM-1 Antibody (Clone W-CAM-1)

#

• Catalog number
  A1552-100
• Price:

  488.15 USD

  Ask for price
• Size
  100 µg

# #

• Antibody Target
  CD54 / ICAM-1
• Target Alternative Name
  CD54; Cell surface glycoprotein P3.58; Human rhinovirus receptor; ICAM-1; Intercellular adhesion molecule 1; Ly 47; Major group rhinovirus receptor; MALA2; MyD10; Surface antigen of activated B cells
• Antibody Type
  Monoclonal
• Clone
  W-CAM-1; same as Wehi-CAM-1 or 1H4
• Species Reactivities
  Human
• Immunogen Sequence
  Raji Burkitt lymphoma cells
• Accession
  P05362
• Gene Id
  3383
• Appearance
  Colorless liquid
• Form
  Liquid
• Formulation
  In 10 mM PBS with 0.05% BSA & 0.05% azide
• Purification
  Protein A/G purification
• Application
  FC, IF, IHC
• Positive Control
  IHC: human Melanoma
• Application And Usages
  FC: 0.5-1 µg/1X106 cells in 0.1 ml, IF: 0.5-1 µg/ml, IHC: 2-4 µg/ml for 30 minutes at RT
• Description
  Recognizes an 85-115 kDa protein (variation with cell type), identified as intercellular adhesion molecule (ICAM-1). It has 7 potential N-linked glycosylation sites. ICAM-1 is a single chain glycoprotein of Ig supergene family, present on unstimulated endothelial cells (EC) and on a variety of other cell types including activated fibroblasts, EC, macrophages, and lymphocytes. ICAM-1 mediates cell adhesion by binding to integrins CD11a/CD18 (leukocyte adhesion molecule, LFA-1) and to CD11b/CD18 (Mac-1). This interaction enhances antigen-specific T-cell activation. ICAM-1 also binds to CD43 and to Plasmodium falciparum infected RBCs. W-CAM-1 mAb blocks aggregation of cell lines mediated by the ICAM-1 and blocks homotypic binding of purified populations of activated T- and B-lymphocytes and also aggregation of mixed T- and B-cell blasts. It inhibits T-cell adhesion to normal human endothelial cells. Activation induced by cell-cell contact (mixed lymphocyte reaction, T-cell mediated B-cell activation) is significantly inhibited. This mAb blocks elements of both effector arms of immune system (cytotoxic cell function and Ig production).
• Storage Condition
  -20 °C
• Shipping Condition
  gel pack
• Shelf Life
  12 months
• Usage
  For Research Use Only! Not to be used in humans
• Handling
  The antibody solution should be gently mixed before use
• Western Blot Verified
  Not Verified
• Immunocytochemistry Verified
  Verified
• Immunofluorescence Verified
  Not Verified
• Immunoprecipitation Verified
  Not Verified
• FACS Verified
  Not Verified
• ELISA Verified
  Not Verified
• ChIP Verified
  Not Verified
• Dot Blot Verified
  Not Verified
• Flow Cytometry Verified
  Not Verified
• Data Sheet Link
• Additional description
  This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
• Properties
  If you buy Antibodies supplied by Biovision they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
• French translation
  anticorps

#

• Gene target
  CD54   ICAM-1   W-CAM-1  
• Gene symbol
  ICAM1, ERVW-1, IGHVII-1-1, MIR1-1, TRUND-NNN8-1, CFAP91, TRUND-NNN7-1, KRIT1, CAMK2N2, ERVFRD-1
• Short name
  Anti-CD54 / ICAM-1 Antibody (Clone W-CAM-1)
• Technique
  Antibody, anti-, anti, antibody to, antibodies, antibodies against human proteins, antibodies for
• Isotype
  Mouse / IgG2b, kappa
• Alternative name
  Antibody toCD54 / ICAM-1 (antibody to-) (clonality W-CAM-1)
• Alternative technique
  antibodies
• Clone name
  W-CAM-1

Gene info

• Identity
  HGNC:5344
• Gene
  ICAM1
• Long gene name
  intercellular adhesion molecule 1
• Synonyms
  • BB2
  • CD54
• Synonyms name
  • human rhinovirus receptor
• Locus
  19p13.2
• Discovery year
  1989-04-24
• Entrez gene record
  3383
• Pubmed identfication
  • 2453850
  • 3871395
• Classification
  • Ig-like cell adhesion molecule family
  • Immunoglobulin like domain containing
  • Receptor ligands
  • CD molecules
• VEGA ID
  OTTHUMG00000180403

Gene info

• Identity
  HGNC:13525
• Gene
  ERVW-1
• Long gene name
  endogenous retrovirus group W member 1, envelope
• Synonyms gene
  • ERVWE1
• Synonyms gene name
  • endogenous retroviral family W, env(C7), member 1
  • endogenous retrovirus group W, member 1
  • endogenous retrovirus group W member 1
• Synonyms
  • HERV-W
  • HERV-W-ENV
  • HERVW
  • HERV-7q
  • envW
• Synonyms name
  • envelope protein
  • HERV-W Env glycoprotein
  • enverin
  • syncytin-1
  • HERV-tryptophan envelope protein
  • HERV-W{7q21.1} provirus ancestral Env polyprotein
  • HERV-7q envelope protein
  • envelope glycoprotein
  • syncytin
• GenBank acession
  • AF208161
• Locus
  7q21.2
• Discovery year
  2000-09-20
• Entrez gene record
  30816
• Pubmed identfication
  • 9835022
  • 9882319
  • 21542922
• RefSeq identity
  • NM_014590
• VEGA ID
  OTTHUMG00000131249

Gene info

• Identity
  HGNC:5670
• Gene
  IGHVII-1-1
• Long gene name
  immunoglobulin heavy variable (II)-1-1 (pseudogene)
• Synonyms gene name
  • immunoglobulin heavy variable (II)-1-1
  • immunoglobulin heavy variable (II)-1-1 pseudogene
• Synonyms
  • IGHV(II)-1-1
• GenBank acession
  • AB019441
• Locus
  14q32.33
• Discovery year
  2000-04-17
• Entrez gene record
  28377
• RefSeq identity
  • NG_001019
• Classification
  • Immunoglobulin heavy locus at 14q32.33
• VEGA ID
  OTTHUMG00000152325

Gene info

• Identity
  HGNC:31499
• Gene
  MIR1-1
• Long gene name
  microRNA 1-1
• Synonyms gene
  • MIRN1-1
• Synonyms
  • hsa-mir-1-1
• Locus
  20q13.33
• Discovery year
  2004-04-23
• Entrez gene record
  406904
• RefSeq identity
  • NR_029780
• Classification
  • MicroRNA MIR1/206 family

Gene info

• Identity
  HGNC:50820
• Gene
  TRUND-NNN8-1
• Long gene name
  tRNA-undetermined (NNN) 8-1
• Synonyms gene name
  • transfer RNA-undetermined (NNN) 8-1
• GenBank acession
  • HG984097
• Locus
  1
• Discovery year
  2014-06-20
• Entrez gene record
  103482528
• Pubmed identfication
  • 18984615

Gene info

• Identity
  HGNC:24010
• Gene
  CFAP91
• Long gene name
  cilia and flagella associated protein 91
• Synonyms gene
  • C3orf15
  • MAATS1
• Synonyms gene name
  • chromosome 3 open reading frame 15
  • MYCBP/AMY-1-associated, testis expressed 1
  • MYCBP-associated, testis expressed 1
  • MYCBP associated and testis expressed 1
• Synonyms
  • AAT1
  • AAT1alpha
  • SPATA26
  • CaM-IP2
  • AAT-1
• Synonyms name
  • AMY-1-associating protein expressed in testis 1
  • MYCBP-binding protein
  • spermatogenesis associated 26
• GenBank acession
  • AB063296
• Locus
  3q13.33
• Discovery year
  2004-07-26
• Entrez gene record
  89876
• Pubmed identfication
  • 12223483
  • 14551891
  • 17967944
• RefSeq identity
  • NM_033364
• VEGA ID
  OTTHUMG00000159422

Gene info

• Identity
  HGNC:50819
• Gene
  TRUND-NNN7-1
• Long gene name
  tRNA-undetermined (NNN) 7-1
• Synonyms gene name
  • transfer RNA-undetermined (NNN) 7-1
• GenBank acession
  • HG984096
• Locus
  1
• Discovery year
  2014-06-20
• Entrez gene record
  103482531
• Pubmed identfication
  • 18984615

Gene info

• Identity
  HGNC:1573
• Gene
  KRIT1
• Long gene name
  KRIT1 ankyrin repeat containing
• Synonyms gene
  • CCM1
• Synonyms gene name
  • cerebral cavernous malformations 1
  • KRIT1, ankyrin repeat containing
• Synonyms
  • CAM
• GenBank acession
  • AJ294850
• Locus
  7q21.2
• Discovery year
  1995-06-02
• Entrez gene record
  889
• Pubmed identfication
  • 7604043
  • 11342228
• Classification
  • FERM domain containing
  • Ankyrin repeat domain containing
  • CCM adhesion complex
  • MicroRNA protein coding host genes
• VEGA ID
  OTTHUMG00000131187
• Locus Specific Databases
  • LRG_650

Gene info

• Identity
  HGNC:24197
• Gene
  CAMK2N2
• Long gene name
  calcium/calmodulin dependent protein kinase II inhibitor 2
• Synonyms
  • CaM-KIIN
• GenBank acession
  • AY037149
• Locus
  3q27.1
• Discovery year
  2005-06-10
• Entrez gene record
  94032
• Pubmed identfication
  • 11444830
  • 9724800
• RefSeq identity
  • NM_033259
• VEGA ID
  OTTHUMG00000156821

Gene info

• Identity
  HGNC:33823
• Gene
  ERVFRD-1
• Long gene name
  endogenous retrovirus group FRD member 1, envelope
• Synonyms gene name
  • endogenous retrovirus group FRD, member 1
  • endogenous retrovirus group FRD member 1
• Synonyms
  • HERV-W/FRD
  • HERV-FRD
  • envFRD
  • ERVFRDE1
  • syncytin-2
• Synonyms name
  • syncytin 2
• GenBank acession
  • AK075092
  • AK123938
  • AY358244
• Locus
  6p24.2
• Discovery year
  2011-05-05
• Entrez gene record
  405754
• Pubmed identfication
  • 12970426
  • 14557543
  • 15476554
  • 21542922
• RefSeq identity
  • NM_207582
• VEGA ID
  OTTHUMG00000159193

MeSH Data

• Name
  Blotting, Western
• Concept
  Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
• Tree numbers
  • E05.196.401.143
  • E05.301.300.096
  • E05.478.566.320.200
  • E05.601.262
  • E05.601.470.320.200
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

Product images

Similar products