Adenovirus Purification Kit

• Catalog number
  K1459-100
• Price
  Please ask
• Size
  100 Preps

• Description
  Magnetic beads based kits
• Summary
  BioVision’s Adenovirus Purification Kit is used to capture, concentrate and store adenoviruses using magnetic beads. Viruses are important gene delivery tools, which have been used in gene therapy and RNAi delivery. Traditionally viruses are purified by ultracentrifugation using CsCl which is time consuming and limited to the volume of cell lysate to be processed. BioVision’s Purification kit uses magnetic nanoparticles to capture viruses by electrostatic and hydrophobic interactions with 80-99% efficiency and the purification process does not need ultracentrifugation. The viruses captured on Adenovirus (AD)-magnetic beads can be used directly for multiple assays. Alternatively, viruses can be eluted with the elution buffer and used for downstream applications.
• Detection Method
  Magnetic beads based virus purification
• Species Reactivity
  All
• Applications
  PCR, western blot, ELISA, transfection directly with viruses bound to magnetic beads or eluted from the magnetic beads (free of beads).
• Sample Type
  Cell and tissue culture supernatants, Serum
• Features Benefits
  Does not need ultracentrifugation, Purify viruses in 30-45 min, High yield and fast concentration (2 to 1000X), Ideal for cell culture transduction/infection
• Storage Conditions
  4°C
• Shipping Conditions
  RT
• Shelf life
  12 months
• Background
  BioVision’s Adenovirus Purification Kit is used to capture, concentrate and store adenoviruses using magnetic beads. Viruses are important gene delivery tools, which have been used in gene therapy and RNAi delivery. Traditionally viruses are purified by ultracentrifugation using CsCl which is time consuming and limited to the volume of cell lysate to be processed. BioVision’s Purification kit uses magnetic nanoparticles to capture viruses by electrostatic and hydrophobic interactions with 80-99% efficiency and the purification process does not need ultracentrifugation. The viruses captured on Adenovirus (AD)-magnetic beads can be used directly for multiple assays. Alternatively, viruses can be eluted with the elution buffer and used for downstream applications. The AD-magnetic beads can be combined with any adenoviruses such as Ad Type 5 (Ad5), Oncolytic Ad (Ad520).
• Data Sheet Link
• Test
  Antibodies are affinity purified with an antigen coated column or protein A or G agarose or beads. DNA is purified with endotoxin free silica columns or anion exchange resins. Biovision supplies purification kits and ultra pure reagents.

• Gene target
  Adenovirus   Kit  
• Short name
  Adenovirus Purification Kit
• Technique
  Purification, purified
• Alternative name
  Adenovirus purification reagent
• Alternative technique
  purifications, kits
• Alternative to gene target
  v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, transferase activity, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832
• Virus
  adenovirus

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