Aconitase Activity Colorimetric Assay Kit

• Catalog number
  K716-100
• Price
  Please ask
• Size
  100 assays

• Description
  Highly Sensitive Assay, HTS
• Summary
  • Detection method- Absorbance (450 nm) • Species reactivity- Mammalian
• Sample Type
  Cell and tissue culture supernatants, urine, plasma and serum, as well as many other biological fluids, fermentation media, food samples, etc.
• Features Benefits
  • Simple procedure; takes ~ less than 40 minutes • Fast and convenient • Sensitive assays for measuring Aconitase activity in various biological samples.
• Storage Conditions
  4°C
• Shipping Conditions
  gel pack
• Shelf life
  12 months
• Background
  Aconitase (aconitate hydratase; EC 4.2.1.3) is an iron-sulfur protein containing an [Fe₄S₄]²ᶧ cluster that catalyzes the stereospecific isomerization of citrate to isocitrate via cis-aconitate in the tricarboxylic acid cycle, a non-redox-active process. Tissue contains two aconitases, a mitochondrial (m-) and a cytosolic (c-) aconitase. They are related, but distinctly different enzymes and are coded for on different chromosomes. Loss of aconitase activity in cells or other biological samples treated with pro-oxidants has been interpreted as a measure of oxidative damage. BioVision’s Aconitase Assay Kit is a highly sensitive, simple, direct and HTS-ready colorimetric assay for measuring Aconitase activity in biological samples. In the assay, citrate is converted by aconitase into isocitrate, which is further processed resulting in a product that converts a nearly colorless probe into an intensely colored form with a λmax at 450nm.
• Data Sheet Link
• Test
  Biovision supplies other types of Assays as 1.
• Properties
  Colorimetric assays or detection use UV absorption or enzymatic color reaction.

• Gene target
  Aconitase   Activity   Kit  
• Short name
  Aconitase Activity Colorimetric Assay Kit
• Technique
  colorimetric, Assay, assays, colorimetry
• Host
  Assay
• Alternative name
  Aconitase Activity colorimetry test reagent
• Alternative technique
  assays, arrays, kits
• Alternative to gene target
  v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, transferase activity, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832

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