3D Cell Culture HTS Cell Viability Complete Assay Kit

• Catalog number
  K948-100
• Price
  Please ask
• Size
  100 assays

• Description
  Highly sensitive
• Summary
  • Detection method- Fluorescence (Ex/Em = 485/530 nm) • Applications- Matrix and spheroid dissociations from 3D cell culture for cell growth assessment - Measurement of cell viability in response to growth factors, cytokines, mitogens and nutrients - • Analysis of cytotoxic/cytostatic compounds that affect cell growth and spheroid formation, such as anticancer drugs, toxic agents and other pharmaceuticals
• Detection Method
  Fluorescence (Ex/Em = 485/530 nm)
• Species Reactivity
  Mammalian
• Applications
  • Matrix and spheroid dissociations from 3D cell culture for cell growth assessment • Measurement of cell viability in response to growth factors, cytokines, mitogens and nutrients • Analysis of cytotoxic/cytostatic compounds that affect cell growth and spheroid formation, such as anticancer drugs, toxic agents and other pharmaceuticals
• Sample Type
  Proliferating and non-proliferating cells in 3D culture Matrices and Scaffolds
• Features Benefits
  • Convenient & non-radioactive • High-throughput • Highly-sensitive: detect as low as 50 viable cells in less than 30 min. • Suitable for cell proliferation, cell viability, chemotaxis, cytotoxicity and apoptosis
• Storage Conditions
  -20°C
• Shipping Conditions
  gel pack
• Shelf life
  12 months
• Background
  Three dimensional (3D) cell cultures are artificially-created environments in which cells are permitted to grow or interact with their surroundings in a 3D fashion. 3D cell cultures improve the function, differentiation and viability of cells and recapitulate in vivo microenvironment compared to conventional 2D cell cultures. 3D matrices provide a physiologically relevant screening platform, by mimicking the in vivo responses, for many cell types including cancer and stem cells in developmental morphogenesis, pharmacology, drug metabolism and drug toxicity studies. Quantification of number of viable cells is an indispensable tool in in vitro screening in these studies. Calcein AM is a non-fluorescent, hydrophobic compound that easily penetrates intact and live cells, and has been widely used to assess cell viability and proliferation in Cell Biology research. However, with the use of 3D matrices, some proteases-based dissociation methods don’t completely dissolve the matrices and cell aggregates, which could alter the result in quantitative in vitro assays such as viability assessment. BioVision’s 3D Cell Culture Cell Viability Complete Assay Kit provides a standardized fluorometric method for sensitive quantification of viable cells that can detect as low as 50 viable cells in each well and can be measured at Ex/Em = 485/530 nm. The measured fluorescence intensity is proportional to the number of viable cells. Further, as a complete set, the kit comes with an optimized and gentle non-enzymatic dissociation solution for the recovery of viable and dead cells from spheroids in matrices and scaffolds. This assay kit provides an easy-to-use, non-radioactive, and high-throughput method for characterizing and screening cell viability, cytotoxicity and apoptosis.
• Data Sheet Link
• Test
  Biovision supplies other types of Assays as 1.
• Additional description
  For cells, cell lines and tissues in culture till half confluency.

• Gene target
  Culture   HTS   Viability   Complete   Kit  
• Short name
  3D Culture HTS Viability Complete Assay Kit
• Technique
  culture, Assay, HTS, assays
• Host
  Assay
• Alternative name
  3D cellular Culture HTS cellular cytotoxity Complete test reagent
• Alternative technique
  arrays, kits
• Alternative to gene target
  v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, transferase activity, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832
• Tissue
  cell

MeSH Data

• Name
  Culture Techniques
• Concept
  Scope note: Methods of maintaining or growing biological materials in controlled laboratory conditions. These include the cultures of CELLS; TISSUES; organs; or embryo in vitro. Both animal and plant tissues may be cultured by a variety of methods. Cultures may derive from normal or abnormal tissues, and consist of a single cell type or mixed cell types.
• Tree numbers
  • E05.481.500
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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