Rabbit anti-Mouse Collagen Type I BrCN Fragment (IgG), polyclonal

  • Catalog number
    31 410 114
  • Price
    Please ask
  • Size
    100 µg/ 100 µl
  • Antibody category
    Polyclonal Antibody
  • Antibody s description
    The antibody was raised against collagen type I BrCN fragment from fetal mouse skin (FMS). The IgG fraction was purified from rabbit neat serum by affinity chromatography.
  • Raised in
    Rabbit
  • Research area of interest
    Diseases associated with collagen I: osteogenesis imperfecta, Ehlers-Danlos Syndrome, Infantile cortical hyperostosis aka Caffey's disease
  • Package form
    liquid
  • Clonality
    Polyclonal
  • Antigen
    collagen type I BrCN fragment from fetal mouse skin (FMS)
  • Reacts with
    rat, mouse
  • Immunogen
    See the data sheet or contact us
  • Contents
    PBS
  • Purity
    >95%
  • Tested applications
    • EIA, ELISA, • Flow Cytometry • Immunoblotting, suggested dilution 2 µg/ml-8 µg/ml • Immunofluorescence • Immunohistochemistry • Immunoprecipitation
  • Other names
    The antibody was raised against collagen type I BrCN fragment from fetal mouse skin (FMS). The IgG fraction was purified from rabbit neat serum by affinity chromatography.
  • Crossreactivity
    weak with bovine and very moderately reactivity with human and weak with bovine collagen type IX. No cross reactivity with collagen type II.
  • Antibody specificity
    reacts with collagen type I of rat and mouse origin. It exhibits weak with bovine and very moderately reactivity with human and weak with bovine collagen type IX. No cross reactivity with collagen type II
  • Purification
    Protein A purified
  • PubMed citations
    See the data sheet
  • UniProt number
    N/A
  • Storage conditions
    - 70°C
  • Technical datasheet
    Contact us to receive the datasheet
  • Shipping condition
    dry ice
  • Warnings
    For Research Use only.
  • Description
    This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
  • Gene
    Collagen Types in bones and muscles can be hydrolyzed and used in human grafts. ( Type II )
  • About
    Immunoglobulin gamma, IgG, mouse monoclonal H&L chain clones or rabbit, goat polyclonal antibodies have 4 parts. There are 2 heavy chains, 2 light chains. The IgG antibody has 2 antigen binding sites. They represent 70% or more of serum antibodies. This antibody can be antigen purified or protein A or G purified. For storage sodium azide is added or you can call us to request azide free antibody preparations. These will need colder storage temperatures. Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera. Rabbits are used for polyclonal antibody production by biotez. Rabbit antibodies are very stable and can be stored for several days at room temperature. biotez adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
  • Test
    Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
  • Latin name
    Mus musculus, Oryctolagus cuniculus
  • Group
    Polyclonals and antibodies
  • Gene target
    Collagen   BrCN  
  • Short name
    Rabbit anti-Mouse Collagen I BrCN (IgG), polyclonal
  • Technique
    Polyclonal, Rabbit, anti-, Mouse, anti, IgG, antibody to, antibodies, IgGs, mouses, Polyclonal antibodies (pAbs) are mostly rabbit or goat antibodies that are secreted by different B cells, whereas monoclonal antibodies come from a single N cell lineage. Pabs are a collection of immunoglobulin molecules that react against a specific antigen, each identifying a different epitope.
  • Host
    Rabbit, Rabbits
  • Isotype
    IgG, Antibody fragments and scFv fragments, single chain variable fragments can be E. coli expressed. Fragment peptides for polyclonals give monoclonal like antibodies as the epitope is very small. IgG Fc and(ab)2 fragments are small and stable subunits of the antibody obtained by enzymatic digestion. Complement fragments, bp DNA fragments are common. IgG
  • Label
    not available conjugated
  • Species
    Mouse, Mouses
  • Alternative name
    production species: rabbit Antibody toMouse Collagen classification I BrCN Fragment (Immunoglobulin G), polyclonal
  • Alternative technique
    polyclonals, rabbit-anti, murine, antibodies, immunoglobulins
MeSH Data
  • Name
  • Concept
    Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
  • Tree numbers
    • E05.196.401.143
    • E05.301.300.096
    • E05.478.566.320.200
    • E05.601.262
    • E05.601.470.320.200
  • Qualifiers
    ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
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