Mouse anti-TNF (IgG), monoclonal

  • Catalog number
    12 500 010
  • Price
    Please ask
  • Size
    1 mg lyophilized
  • Antibody category
    Monoclonal Antibody
  • Antibody s description
    Monoclonal antibody to human TNF. Immunoglobulin isotype: IgG2a. The monoclonal antibody was raised against recombinant human tumor necrose factor alpha (TNFa). The immune lymphocytes from BALB/c mice were extracted from lymph nodes and fused with myeloma sp2/0 cells according to a standard protocol. The IgG1 fraction was purified by Protein G affinity chromatography. The antibody has been reported for use in detection of human TNF alpha by ELISA. It can also be used for immunoblotting.
  • Raised in
    mouse
  • Research area of interest
    Autoimmune disease
  • Package form
    lyophilized
  • Clonality
    Monoclonal
  • Antigen
    recombinant human TNF
  • Reacts with
    Human
  • Immunogen
    See the data sheet or contact us
  • Contents
    PBS, with 0,1% Azide
  • Purity
    >95% pure. Purity is tested by electrophoresis.
  • Tested applications
    • ELISA • Immunoblotting
  • Other names
    Mab to human TNF. Immunoglobulin isotype: IgG2a. The monoclonal antibody was raised against recombinant human tumor necrose factor alpha (TNFa). The immune lymphocytes from BALB/c mice were extracted from lymph nodes and fused with myeloma sp2/0 cells according to a standard protocol. The IgG1 fraction was purified by Protein G affinity chromatography. The antibody has been reported for use in detection of human TNF alpha by ELISA. It can also be used for immunoblotting.
  • Crossreactivity
    no crossreactivty
  • Antibody specificity
    recombinant and human TNF
  • Purification
    Protein G purified
  • PubMed citations
    1. Strohner P: Patent: EP 1957980B1 Immunoassay for the simultaneous immunochemical determination of an analyte (antigen) and a treatment antibody targeting the analyte in samples (Recovery immunoassay) 2. Korn S, Haasler I, Jung M, Strohner P, Staatz A, Schäfer A, Becher G, Taube C and Buhl R Free serum IgE in patients with severe allergic asthma treated with Omalizumab Am. J. Respir. Crit. Care Med., May 2010; 181: A6655 3. Strohner P, Sarrach D, Reich JG, Becher G, Staatz A, Schäfer A, Steiß J-O, Häupl T: The Recovery-ELISA – a novel Assay Technique for the Therapy Control of Therapeutic Antibodies Am J Respir Crit Care Med 181;2010:A5674 4. Strohner P, Staatz A, Schäfer A, Becher G, Sarrach D, Reich JG, Steiß J-O, Häupl T: Monitoring of Monoclonal Antibody Therapy with a new Recovery ELISA Assay Technique (R-ELISA®) ERS Barcelona 2010, Abstract-No: 3399 5. Steiß JO, Schmidt A, Lindemann H, Rudloff S, Staatz A, Strohner P, Becher G, Nährlich L, Zimmer KP: Therapiemonitoring von Omalizumab im Kindes- und Jugendalter. Allergologie, Jahrgang 33, Nr. 10/2010, S. 421–428
  • UniProt number
    P01377
  • Storage conditions
    -20°C long term; +4°C short term
  • Technical datasheet
    Contact us to receive the datasheet
  • Shipping condition
    -80°C +37°C
  • Warnings
    For Research Use only.
  • Description
    This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
  • About
    Immunoglobulin gamma, IgG, mouse monoclonal H&L chain clones or rabbit, goat polyclonal antibodies have 4 parts. There are 2 heavy chains, 2 light chains. The IgG antibody has 2 antigen binding sites. They represent 70% or more of serum antibodies. This antibody can be antigen purified or protein A or G purified. For storage sodium azide is added or you can call us to request azide free antibody preparations. These will need colder storage temperatures. Monoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes.
  • Test
    Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
  • Latin name
    Mus musculus
  • Gene
    Tumor necrosis factor (TNFa, tumor necrosis factor alpha, TNFα, cachexin, or cachectin) is a cell signaling protein (cytokine) involved in systemic inflammation and is one of the cytokines that make up the acute phase reaction. It is produced chiefly by activated macrophages, although it can be produced by many other cell types such as CD4+ lymphocytes, NK cells, neutrophils, mast cells, eosinophils, and neurons. TNFb or TNF beta also bin on TNF receptors for Th1 activation.
  • Gene target
    TNF   monoclonal  
  • Gene symbol
    TNF, TNFRSF1A, TNFRSF1B, LTBR
  • Short name
    Mouse anti-TNF (IgG), monoclonal
  • Technique
    anti-, Mouse, anti, IgG, antibody to, antibodies, IgGs, Monoclonals or monoclonal antibodies, mouses
  • Host
    mouse
  • Isotype
    IgG2a, IgG
  • Label
    with label on request, e.g. Biotin, HRP
  • Species
    Mouse, Mouses
  • Alternative name
    Mouse Antibody toTNF (Immunoglobulin G), monoclonal
  • Alternative technique
    murine, antibodies, immunoglobulins
  • Alternative to gene target
    tumor necrosis factor, DIF and TNF-alpha and TNFA and TNFSF2, TNF and IDBG-300259 and ENSG00000232810 and 7124, transcription regulatory region DNA binding, Extracellular, Tnf and IDBG-177358 and ENSMUSG00000024401 and 21926, TNF and IDBG-634161 and ENSBTAG00000025471 and 280943
Gene info
Gene info
Gene info
Gene info
MeSH Data
  • Name
  • Concept
    Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
  • Tree numbers
    • E05.196.401.143
    • E05.301.300.096
    • E05.478.566.320.200
    • E05.601.262
    • E05.601.470.320.200
  • Qualifiers
    ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
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