Rabbit Anti-Guinea Pig IgM Antibody (H+L), Biotin Conjugated

• Catalog number
  bs-0375R-Biotin
• Price
  Please ask
• Size
  0.2ml

• Category
  Conjugated Secondary Antibody
• Conjugation
  Biotin
• Host Organism
  Rabbit
• Target antigen
  Guinea Pig IgM
• Modification
  Unmodified
• Modification site
  None
• Clonality
  Polyclonal antibody
• Clone
  Polyclonal antibody
• Concentration
  1ug/ul
• Tested applications
  WB, ELISA, IHC-P, IHC-F
• Recommended dilutions
  WB(1:500-2000), ELISA(1:500-2000), IHC-P(1:500-2000), IHC-F(1:500-2000)
• Cross reactivity
  Guinea Pig
• Background of the target antigen
  Immunoglobulin IgM normally constitutes about 10% of serum immunoglobulins. IgM antibody is prominent in early immune responses to most antigens and is largely confined to plasma due to it's large size. Monomeric IgM is expressed as a membrane bound antibody on the surface of B cells and as a pentamer when secreted by plasma cells.
• Purification method
  This antibody has been purified via Protein A.
• Storage conditions
  Keep the antibody in aqueous buffered solution containing 1% BSA, 50% glycerol and 0.09% sodium azide. Store at -20°C for up to one year.
• Description
  This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use. Guinea pig ELISA kits for plasma and sera samples are used to study human genes through the guinea pig model (Cavia porcellus), also called the cavy rodent model. After mouzes and rats Guinea pigs are easy in maintained laboratory animals. cDNAs of Guinea pigs are also very popular.
• Properties
  If you buy Antibodies supplied by Bioss Secondary Antibodies they should be stored frozen at - 24°C for long term storage and for short term at + 5°C. Biotin conjugates can be detected by horseradish peroxidase, alkaline phosphatase substrates or anti biotin conjugated antibodies. Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA. To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1.6. Immunoglobulin M, or IgM for short, is a basic antibody that is produced by B cells. IgM is by far the physically largest antibody in the human circulatory system. It is the first antibody to appear in response to initial exposure to an antigen
• Additional conjugation
  Biotinylated
• About
  Pigs and the smaller guinea pigs are frequent used as models for humans. Rabbits are used for polyclonal antibody production by Bioss Secondary Antibodies. Rabbit antibodies are very stable and can be stored for several days at room temperature. Bioss Secondary Antibodies adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
• Latin name
  Oryctolagus cuniculus
• French translation
  anticorps

• Gene target
  H+L   Conjugated  
• Short name
  Anti-Guinea Pig IgM
• Technique
  Antibody, Rabbit, anti-, anti, IgM, antibody to, antibodies, antibodies against human proteins, antibodies for, antibody Conjugates, IgMs
• Host
  Rabbit, Rabbits
• Isotype
  IgM, IgM
• Label
  Biotin
• Species
  Guinea Pig, Guinea Pigs, Pigs and Guinea pigs
• Alternative name
  Rabbit Anti-Guinea Pig IgM
• Alternative technique
  antibodies, rabbit-anti, immunoglobulin-m

MeSH Data

• Name
  Blotting, Western
• Concept
  Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
• Tree numbers
  • E05.196.401.143
  • E05.301.300.096
  • E05.478.566.320.200
  • E05.601.262
  • E05.601.470.320.200
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

Product images

Similar products