Goat Anti-Mouse IgM Antibody (H+L), Biotin Conjugated

• Catalog number
  bs-0368G-Biotin
• Price
  Please ask
• Size
  0.2ml

• Category
  Conjugated Secondary Antibody
• Conjugation
  Biotin
• Host Organism
  Goat
• Target antigen
  Mouse IgM
• Modification
  Unmodified
• Modification site
  None
• Clonality
  Polyclonal antibody
• Clone
  Polyclonal antibody
• Concentration
  1ug/ul
• Tested applications
  WB, ELISA, IHC-P, IHC-F
• Recommended dilutions
  WB(1:500-2000), ELISA(1:500-2000), IHC-P(1:500-2000), IHC-F(1:500-2000)
• Cross reactivity
  Mouse
• Background of the target antigen
  Immunoglobulin IgM normally constitutes about 10% of serum immunoglobulins. IgM antibody is prominent in early immune responses to most antigens and is largely confined to plasma due to it's large size. Monomeric IgM is expressed as a membrane bound antibody on the surface of B cells and as a pentamer when secreted by plasma cells.
• Purification method
  This antibody has been purified via Protein A.
• Storage conditions
  Keep the antibody in aqueous buffered solution containing 1% BSA, 50% glycerol and 0.09% sodium azide. Store at -20°C for up to one year.
• Description
  This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
• Properties
  If you buy Antibodies supplied by Bioss Secondary Antibodies they should be stored frozen at - 24°C for long term storage and for short term at + 5°C. Biotin conjugates can be detected by horseradish peroxidase, alkaline phosphatase substrates or anti biotin conjugated antibodies. Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA. To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1.6. Immunoglobulin M, or IgM for short, is a basic antibody that is produced by B cells. IgM is by far the physically largest antibody in the human circulatory system. It is the first antibody to appear in response to initial exposure to an antigen
• Additional conjugation
  Biotinylated
• Latin name
  Capra aegagrus hircus, Mus musculus
• Test
  Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
• French translation
  anticorps

• Gene target
  H+L   Conjugated  
• Short name
  Anti-Mouse IgM
• Technique
  Antibody, anti-, Mouse, anti, Goat, IgM, antibody to, antibodies, antibodies against human proteins, antibodies for, antibody Conjugates, goats, IgMs, mouses
• Host
  Goat, The goat polyclonal antibodies are directed against human genes or rabbit or mouse IgGs (H +L) chain primary antibodies and conjugated for detection. It are very good secondary antibodies that are affinity purified and have a good affinity themselves too. Often KLH is coupled to the antigen so anti KLH can be present in the vial.
• Isotype
  IgM, IgM
• Label
  Biotin
• Species
  Mouse, Goats, Mouses
• Alternative name
  Goat Anti-Mouse IgM
• Alternative technique
  antibodies, murine, caprine, immunoglobulin-m

MeSH Data

• Name
  Blotting, Western
• Concept
  Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
• Tree numbers
  • E05.196.401.143
  • E05.301.300.096
  • E05.478.566.320.200
  • E05.601.262
  • E05.601.470.320.200
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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