Chicken IgY Polyclonal Antibody, FITC Conjugated

  • Catalog number
    bs-0432R-FITC
  • Price
    Please ask
  • Size
    0.2ml
  • Category
    Conjugated Secondary Antibody
  • Conjugation
    FITC
  • Host Organism
    Rabbit
  • Target antigen
    Chicken IgY
  • Modification
    Unmodified
  • Modification site
    None
  • Clonality
    Polyclonal antibody
  • Clone
    Polyclonal antibody
  • Concentration
    1ug/ul
  • Tested applications
    IF(IHC-P)
  • Recommended dilutions
    IF(IHC-P)(1:500-2000)
  • Cross reactivity
    Chicken
  • Background of the target antigen
    In chickens, immunoglobulin Y is the functional equivalent to Immunoglobulin G (IgG). Like IgG, it is composed of two light and two heavy chains. Structurally, these two types of immunoglobulin differ primarily in the heavy chains, which in IgY have a molecular mass of about 65,100 atomic mass units (amu), and are thus larger than in IgG. The light chains in IgY, with a molar mass of about 18,700 amu, are somewhat smaller than the light chains in IgG. The molar mass of IgY thus amounts to about 167,000 amu. The steric flexibility of the IgY molecule is less than that of IgG.Functionally, IgY is partially comparable to Immunoglobulin E (IgE), as well as to IgG. However, in contrast to IgG, IgY does not bind to Protein A, to Protein G, or to cellular Fc receptors. Furthermore, IgY does not activate the complement system. The name Immunoglobulin Y was suggested in 1969 by G.A. Leslie and L.W. Clem, after they were able to show differences between the immunoglobulins found in chicken eggs, and immunoglobulin G. Other synonymous names are Chicken IgG, Egg Yolk IgG, and 7S-IgG.
  • Purification method
    This antibody has been purified via Protein A.
  • Storage conditions
    Keep the antibody in aqueous buffered solution containing 1% BSA, 50% glycerol and 0.09% sodium azide. Store at -20°C for up to one year.
  • Properties
    If you buy Antibodies supplied by Bioss Secondary Antibodies they should be stored frozen at - 24°C for long term storage and for short term at + 5°C. Chickens like all bird species have IgY antibodies that are very stable and found in the yolk. Gallus Gallus domestica is the Latin name of the Chicken. This Bioss Secondary Antibodies Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. Bioss Secondary Antibodies FITC is highly sensitive to pH extremes. Chicken yolk polyclonal Immunoglobulin Y (abbreviated as IgY) is a type of immunoglobulin which is the major antibody in bird, reptile, and lungfish blood. It is also found in high concentrations in chicken egg yolk. As with the other immunoglobulins, IgY is a class of proteins which are formed by the immune system in reaction to certain foreign substances, and specifically recognize them.
  • Additional conjugation
    Anti-FITC Antibody
  • Group
    Polyclonals and antibodies
  • About
    Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera.
  • French translation
    anticorps
  • Gene target
  • Short name
    Chicken IgY Polyclonal
  • Technique
    Polyclonal, Antibody, FITC, IgY, antibodies against human proteins, antibodies for, antibody Conjugates, Fluorescein, IgYs, Polyclonal antibodies (pAbs) are mostly rabbit or goat antibodies that are secreted by different B cells, whereas monoclonal antibodies come from a single N cell lineage. Pabs are a collection of immunoglobulin molecules that react against a specific antigen, each identifying a different epitope.
  • Isotype
    IgG, IgY antibody
  • Label
    FITC
  • Species
    chicken, Chickens
  • Alternative name
    Chicken IgY Polyclonal
  • Alternative technique
    polyclonals, antibodies, fluorescine, bird
MeSH Data
  • Name
  • Concept
    Scope note: A method for the study of certain organic compounds within cells, in situ, by measuring the light intensities of the selectively stained areas of cytoplasm. The compounds studied and their locations in the cells are made to fluoresce and are observed under a microscope.
  • Tree numbers
    • E01.370.225.500.386
    • E05.196.712.516.600.240
    • E05.200.500.386
    • E05.242.386
  • Qualifiers
    ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
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