TRIM25(Thr91) Antibody, FITC Conjugated

• Catalog number
  bs-5614R-FITC
• Price
  Please ask
• Size
  0.1ml

• Long name
  TRIM25(Thr91) Polyclonal Antibody, FITC Conjugated
• Category
  Conjugated Primary Antibodies
• Conjugation
  FITC
• Host Organism
  Rabbit (Oryctolagus cuniculus)
• Target Antigen
  TRIM25 Thr91
• Specificity
  This is a highly specific antibody against TRIM25 Thr91.
• Modification
  Phosphorylation
• Modification site
  Thr91
• Clonality
  Polyclonal
• Clone
  Polyclonal antibody
• Concentration
  1ug per 1ul
• Source
  KLH conjugated synthetic phosphopeptide derived from human TRIM25 around the phosphorylation site of Thr91
• Gene ID number
  7706
• Tested applications
  IF(IHC-P)
• Recommended dilutions
  IF(IHC-P)(1:50-200)
• Crossreactivity
  Human
• Crossreactive species details
  Due to limited amount of testing and knowledge, not every possible cross-reactivity is known.
• Antigen background
  The protein encoded by this gene is a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc binding domains, a RING, a B box type 1 and a B box type 2, and a coiled coil region. The protein localizes to the cytoplasm. The presence of potential DNA binding and dimerization transactivation domains suggests that this protein may act as a transcription factor, similar to several other members of the TRIM family. Expression of the gene is upregulated in response to estrogen, and it is thought to mediate estrogen actions in breast cancer as a primary response gene.
• Purification method
  This antibody was purified via Protein A.
• Storage conditions
  Keep the antibody in an aqueous buffered solution containing 1% BSA, 50% glycerol and 0.09% sodium azide. Store refrigerated at 2 to 8 degrees Celcius for up to 1 year.
• Excitation Emission
  494nm/518nm
• Synonyms
  TRIM25phospho T91ï¼›EFP; Estrogen responsive finger protein; RNF 147; RNF147; TRI25; TRIM 25; Tripartite mot containing 25; Tripartite mot containing protein 25; Z147; Zinc finger protein 147; Zinc finger protein 147 estrogen responsive finger protein; ZNF 147; ZNF147.
• Properties
  If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies they should be stored frozen at - 24°C for long term storage and for short term at + 5°C. This Bioss Primary Conjugated Antibodies Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. Bioss Primary Conjugated Antibodies FITC is highly sensitive to pH extremes.
• Additional conjugation
  Anti-FITC Antibody
• French translation
  anticorps

• Gene target
  TRIM25   Thr91   Conjugated  
• Gene symbol
  TRIM25
• Short name
  Anti-TRIM25 Thr91
• Technique
  Antibody, FITC, antibodies against human proteins, antibodies for, antibody Conjugates, Fluorescein
• Isotype
  Immunoglobulin G (IgG)
• Label
  FITC
• Alternative name
  Anti-TRIM25 Thr91 PAb FITC
• Alternative technique
  antibodies, fluorescine

Gene info

• Identity
  HGNC:12932
• Gene
  TRIM25
• Long gene name
  tripartite motif containing 25
• Synonyms gene
  • ZNF147
• Synonyms gene name
  • zinc finger protein 147 (estrogen-responsive finger protein)
  • tripartite motif-containing 25
• Synonyms
  • EFP
  • RNF147
• GenBank acession
  • D21205
• Locus
  17q22
• Discovery year
  1994-03-16
• Entrez gene record
  7706
• Pubmed identfication
  • 7789997
• RefSeq identity
  • NM_005082
• Classification
  • Tripartite motif containing
  • MicroRNA protein coding host genes
  • Ring finger proteins
• VEGA ID
  OTTHUMG00000178124

MeSH Data

• Name
  Fluorescent Antibody Technique
• Concept
  Scope note: Test for tissue antigen using either a direct method, by conjugation of antibody with fluorescent dye (FLUORESCENT ANTIBODY TECHNIQUE, DIRECT) or an indirect method, by formation of antigen-antibody complex which is then labeled with fluorescein-conjugated anti-immunoglobulin antibody (FLUORESCENT ANTIBODY TECHNIQUE, INDIRECT). The tissue is then examined by fluorescence microscopy.
• Tree numbers
  • E01.370.225.500.607.512.240
  • E01.370.225.750.551.512.240
  • E05.200.500.607.512.240
  • E05.200.750.551.512.240
  • E05.478.583.375
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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