RUNX1 (Ser249) Antibody, Biotin Conjugated

• Catalog number
  bs-3023R-Biotin
• Price
  Please ask
• Size
  0.1ml

• Long name
  RUNX1 (Ser249) Polyclonal Antibody, Biotin Conjugated
• Category
  Conjugated Primary Antibodies
• Conjugation
  Biotin
• Host Organism
  Rabbit (Oryctolagus cuniculus)
• Target Antigen
  RUNX1 Ser249
• Specificity
  This is a highly specific antibody against RUNX1 Ser249.
• Modification
  Phosphorylation
• Modification site
  Ser249
• Clonality
  Polyclonal
• Clone
  Polyclonal antibody
• Concentration
  1ug per 1ul
• Source
  KLH conjugated synthetic phosphopeptide derived from human RUNX1 around the phosphorylation site of Ser249 [QP(p-S)PP]
• Tested applications
  WB, IHC-P
• Recommended dilutions
  WB(1:100-1000), IHC-P(1:100-500)
• Crossreactivity
  Human, Mouse, Rat
• Crossreactive species details
  Due to limited amount of testing and knowledge, not every possible cross-reactivity is known.
• Antigen background
  AML1/Runx1 binds DNA as a monomer and through the Runt domain. DNA binding is increased by heterodimerization with CBFB. Isoform AML1L can neither bind DNA nor heterodimerize and interferes with the transactivation activity of AML1/Runx1. CBF binds to the core site, 5'-PYGPYGGT-3', of a number of enhancers and promoters, including murine leukemia virus, polyomavirus enhancer, T cell receptor enhancers, LCK, IL3 and GMCSF promoters. The alpha subunit binds DNA and appears to have a role in the development of normal hematopoiesis. AML1/Runx1 is expressed in a wide variety of tissues and is expressed at the highest levels in thymus, bone marrow and peripheral blood. Defects in AML1/Runx1 are the cause of familial platelet disorder with associated myeloid malignancy, an autosomal dominant disease characterized by qualitative and quantitative platelet defects, and propensity to develop acute myelogenous leukemia.
• Purification method
  This antibody was purified via Protein A.
• Storage conditions
  Keep the antibody in an aqueous buffered solution containing 1% BSA, 50% glycerol and 0.09% sodium azide. Store refrigerated at 2 to 8 degrees Celcius for up to 1 year.
• Excitation Emission
  N/A
• Synonyms
  RUNX1 Ser249; p-RUNX1 Ser249; RUNX1 phospho S249; RUNX1 phospho Ser249; Acute myeloid leukemia 1; Acute myeloid leukemia 1 protein; alpha subunit core binding factor; AML 1; AML1 EVI 1; AML1; Aml1 oncogene; AMLCR 1; AMLCR1; CBFA 2; CBFA2; Core binding factor alpha 2 subunit; Core binding factor runt domain alpha subunit 2; EVI 1; EVI1; HGNC; Oncogene AML 1; PEA2 alpha; PEBP2 alpha B; PEBP2A2; PEBP2aB; Polyomavirus enhancer binding protein 2 alpha B subunit; Run1; Runt related transcription factor 1; RUNX 1; SL3 3 enhancer factor 1 alpha B subunit; SL3/AKV core binding factor alpha B subunit; RUNX1_HUMAN.
• Properties
  If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies they should be stored frozen at - 24°C for long term storage and for short term at + 5°C. Biotin conjugates can be detected by horseradish peroxidase, alkaline phosphatase substrates or anti biotin conjugated antibodies. Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA. To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1.6.
• Additional conjugation
  Biotinylated
• French translation
  anticorps

• Gene target
  RUNX1   Ser249   Conjugated  
• Gene symbol
  RUNX1-IT1, RUNX1
• Short name
  Anti-RUNX1 Ser249
• Technique
  Antibody, antibodies against human proteins, antibodies for, antibody Conjugates
• Isotype
  Immunoglobulin G (IgG)
• Label
  Biotin
• Alternative name
  Anti-RUNX1 Ser249 PAb Biotin
• Alternative technique
  antibodies
• Alternative to gene target
  runt-related transcription factor 1, AML1 and AML1-EVI-1 and AMLCR1 and CBFA2 and EVI-1 and PEBP2aB, RUNX1 and IDBG-2825 and ENSG00000159216 and 100506403,101928269,861, repressing transcription factor binding, nuclei, Runx1 and IDBG-177713 and ENSMUSG00000022952 and 12394, RUNX1 and IDBG-640443 and ENSBTAG00000004742 and 529631

Gene info

• Identity
  HGNC:16623
• Gene
  RUNX1-IT1
• Long gene name
  RUNX1 intronic transcript 1
• Synonyms gene
  • C21orf96
• Synonyms gene name
  • chromosome 21 open reading frame 96
  • RUNX1 intronic transcript 1 (non-protein coding)
• Synonyms
  • FLJ20856
• GenBank acession
  • AK024509
• Locus
  21q22.12
• Discovery year
  2003-10-01
• Entrez gene record
  80215
• Pubmed identfication
  • 32487998
• RefSeq identity
  • NR_026812
• Classification
  • Intronic transcripts

Gene info

• Identity
  HGNC:10471
• Gene
  RUNX1
• Long gene name
  RUNX family transcription factor 1
• Synonyms gene
  • AML1
  • CBFA2
• Synonyms gene name
  • acute myeloid leukemia 1
  • runt-related transcription factor 1
  • runt related transcription factor 1
• Synonyms
  • PEBP2A2
  • AMLCR1
• Synonyms name
  • aml1 oncogene
• GenBank acession
  • X79549
• Locus
  21q22.12
• Discovery year
  1991-08-20
• Entrez gene record
  861
• Pubmed identfication
  • 1427868
  • 7835892
• Classification
  • Runt-related transcription factors
• VEGA ID
  OTTHUMG00000086299
• Locus Specific Databases
  • LRG_482

MeSH Data

• Name
  Blotting, Western
• Concept
  Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
• Tree numbers
  • E05.196.401.143
  • E05.301.300.096
  • E05.478.566.320.200
  • E05.601.262
  • E05.601.470.320.200
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

Product images

Similar products