ATF2 (Ser480) Antibody, Biotin Conjugated

• Catalog number
  bs-12536R-Biotin
• Price
  Please ask
• Size
  0.1ml

• Long name
  ATF2 (Ser480) Polyclonal Antibody, Biotin Conjugated
• Category
  Conjugated Primary Antibodies
• Conjugation
  Biotin
• Host Organism
  Rabbit (Oryctolagus cuniculus)
• Target Antigen
  ATF2 Ser480
• Specificity
  This is a highly specific antibody against ATF2 Ser480.
• Modification
  Phosphorylation
• Modification site
  Ser480
• Clonality
  Polyclonal
• Clone
  Polyclonal antibody
• Concentration
  1ug per 1ul
• Source
  KLH conjugated synthetic phosphopeptide derived from mouse ATF2 around the phosphorylation site of Ser480
• Gene ID number
  11909
• Tested applications
  WB, IHC-P
• Recommended dilutions
  WB(1:100-1000), IHC-P(1:100-500)
• Crossreactivity
  Human, Mouse
• Crossreactive species details
  Due to limited amount of testing and knowledge, not every possible cross-reactivity is known.
• Antigen background
  ATF2 is a member of the ATF/CREB family of basic region leucine zipper DNA binding proteins that regulates transcription by binding to a consensus cAMP response element (CRE) in the promoter of various viral and cellular genes. Many of these genes are important in cell growth and differentiation, and in stress and immune responses. ATF2 is a nuclear protein that binds DNA as a dimer and can form dimers with members of the ATF/CREB and Jun/Fos families. It is a stronger activator as a heterodimer with cJun than as a homodimer. Several isoforms of ATF2 arise by differential splicing. The stable native full length ATF2 is transcriptionally inactive as a result of an inhibitory direct intramolecular interaction of its carboxy terminal DNA binding domain with the amino terminal transactivation domain. Following dimerization ATF2 becomes a short lived protein that undergoes ubiquitination and proteolysis, seemingly in a protein phosphatase-dependent mechanism. Stimulation of the transcriptional activity of ATF2 occurs following cellular stress induced by several genotoxic agents, inflammatory cytokines, and UV irradiation. This activation requires phosphorylation of two threonine residues in ATF2 by both JNK/SAP kinase and p38 MAP kinase. ATF2 is abundantly expressed in brain.
• Purification method
  This antibody was purified via Protein A.
• Storage conditions
  Keep the antibody in an aqueous buffered solution containing 1% BSA, 50% glycerol and 0.09% sodium azide. Store refrigerated at 2 to 8 degrees Celcius for up to 1 year.
• Excitation Emission
  N/A
• Synonyms
  ATF2 phospho S480; p-ATF2 phospho S480; p-ATF2 phospho Ser480; ATF2Ser480; CREB 2; HB 16; Activating Transcription Factor 2; ATF 2; Atf-2; ATF2 protein; cAMP Response Element Binding Protein 2; cAMP response element binding protein CRE BP1; cAMP-dependent transcription factor ATF-2; cAMP-responsive element-binding protein 2; CRE BP1; CRE-BP; CREB 2; CREB2; CREBP1; Cyclic AMP dependent transcription factor ATF 2; Cyclic AMP-responsive; ATF2_HUMAN.
• Properties
  If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies they should be stored frozen at - 24°C for long term storage and for short term at + 5°C. Biotin conjugates can be detected by horseradish peroxidase, alkaline phosphatase substrates or anti biotin conjugated antibodies. Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA. To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1.6.
• Additional conjugation
  Biotinylated
• French translation
  anticorps

• Gene target
  ATF2   Ser480   Conjugated  
• Gene symbol
  GDNF, ATF2
• Short name
  Anti-ATF2 Ser480
• Technique
  Antibody, antibodies against human proteins, antibodies for, antibody Conjugates
• Isotype
  Immunoglobulin G (IgG)
• Label
  Biotin
• Alternative name
  Anti-ATF2 Ser480 PAb Biotin
• Alternative technique
  antibodies

Gene info

• Identity
  HGNC:4232
• Gene
  GDNF
• Long gene name
  glial cell derived neurotrophic factor
• Synonyms
  • ATF1
  • ATF2
  • HFB1-GDNF
• Synonyms name
  • astrocyte-derived trophic factor
  • glial cell line derived neurotrophic factor
  • glial derived neurotrophic factor
• Locus
  5p13.2
• Discovery year
  1995-05-04
• Entrez gene record
  2668
• Pubmed identfication
  • 8493557
• RefSeq identity
  • NM_000514
• Classification
  • GDNF family ligands
• VEGA ID
  OTTHUMG00000090809

Gene info

• Identity
  HGNC:784
• Gene
  ATF2
• Long gene name
  activating transcription factor 2
• Synonyms gene
  • CREB2
• Synonyms gene name
  • cAMP responsive element binding protein 2
• Synonyms
  • TREB7
  • CRE-BP1
  • HB16
• GenBank acession
  • X15875
• Locus
  2q31.1
• Discovery year
  1991-08-01
• Entrez gene record
  1386
• Pubmed identfication
  • 1833307
  • 1838349
• RefSeq identity
  • NM_001880
• Classification
  • Basic leucine zipper proteins
  • MicroRNA protein coding host genes
• VEGA ID
  OTTHUMG00000132424

MeSH Data

• Name
  Blotting, Western
• Concept
  Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
• Tree numbers
  • E05.196.401.143
  • E05.301.300.096
  • E05.478.566.320.200
  • E05.601.262
  • E05.601.470.320.200
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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