5 MethylCytosine Antibody, Cy3 Conjugated

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• Catalog number
  bs-9450R-Cy3
• Price:

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• Size
  0.1ml

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• Long name
  5 MethylCytosine Polyclonal Antibody, Cy3 Conjugated
• Category
  Conjugated Primary Antibodies
• Conjugation
  Cy3
• Host Organism
  Rabbit (Oryctolagus cuniculus)
• Target Antigen
  5 MethylCytosine
• Specificity
  This is a highly specific antibody against 5 MethylCytosine.
• Modification
  Unmodified
• Modification site
  None
• Clonality
  Polyclonal
• Clone
  Polyclonal antibody
• Concentration
  1ug per 1ul
• Source
  KLH conjugated 5 MethylCytosine
• Tested applications
  IF(IHC-P)
• Recommended dilutions
  IF(IHC-P)(1:50-200)
• Crossreactivity
  Others
• Crossreactive species details
  5 MethylCytosine
• Antigen background
  Cytidine is a nucleoside formed by a cytosine attached to a ribose ring via a beta-N1-glycosidic bond. DNA is methylated on cytidines by DNA methylases (DNMTs)to generate 5-methylcytidine (5-mC), a potent epigenetics marker and regulator of gene expression. The reverse reaction (cytidine demethylation) starts with its oxidation to hydroxymethyl- (5-hmC), formyl- (5-fC), and carboxy- (5-caC) cytidine. Several enzymes, including the Tet family of proteins have been implicated in cytidine demethylation.
• Purification method
  This antibody was purified via Protein A.
• Storage conditions
  Keep the antibody in an aqueous buffered solution containing 1% BSA, 50% glycerol and 0.09% sodium azide. Store refrigerated at 2 to 8 degrees Celcius for up to 1 year.
• Excitation Emission
  512,550nm/570,615nm
• Synonyms
  5-Methyl Cytidine; 5 m C; 5 mC; 5 me C; 5 Me Cytidine; 5 MeCyd; 5 Methyl Cytidine; 5 MethylCytosine; 5-mC; 5-Me Cytidine; 5-Methyl-Cytosine; 5-MethylCytidine.
• Properties
  If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies they should be stored frozen at - 24°C for long term storage and for short term at + 5°C. Cy3 antibodies are excited by the 488-nanometer wave of an argon laser and the 633-nanometer line of a helium-neon diode laser. 1 of the Anti-5 MethylCytosine can be used in flow cytometry but typically shows lower fluorescence intensity comparable to that of PE or APC. This Bioss Primary Conjugated Antibodies antibody is well suited for fluorescent microscopy.
• Additional conjugation
  cy3 conjugation kit
• French translation
  anticorps

#

• Gene target
  MethylCytosine   Conjugated  
• Short name
  Anti-5 MethylCytosine
• Technique
  Antibody, antibodies against human proteins, antibodies for, antibody Conjugates
• Isotype
  Immunoglobulin G (IgG)
• Label
  Cy3
• Alternative name
  Anti-5 MethylCytosine PAb Cy3
• Alternative technique
  antibodies

MeSH Data

• Name
  Blotting, Western
• Concept
  Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
• Tree numbers
  • E05.196.401.143
  • E05.301.300.096
  • E05.478.566.320.200
  • E05.601.262
  • E05.601.470.320.200
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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