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Type
Conjugated Primary Antibody
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Conjugated with
ALEXA FLUOR® 594
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Host organism
Mouse (Mus musculus)
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Target Protein Peptide
KLH
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Specificity
This antibody reacts specifically with KLH
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Modification
No modification has been applied to this antibody
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Modification site
None
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Clonality
Polyclonal Antibody
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Clone
Polyclonal Antibodies
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Concentration
1ug per 1ul
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Subcellular locations
N/A
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Antigen Source
KLH protein
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Gene ID
N/A
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Swiss Prot
N/A
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Applications
IF(IHC-P)
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Applications with corresponding dilutions
IF(IHC-P)(1:50-200)
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Cross reactive species
Others
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Cross Reactive Species details
Keyhole limpet hemocyanin (KLH)
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Background information
Keyhole limpet hemocyanin is an extremely large, heterogeneous glycosylated protein consisting of subunits with a molecular weight of 350,000 and 390,000 in aggregates with molecular weights of 4,500,000-13,000,000. Each domain of a KLH subunit contains two copper atoms that together bind a single oxygen molecule (O2). When oxygen is bound to hemocyanin, the molecule takes on a distinctive transparent, opalescent blue color. The KLH protein is potently immunogenic yet safe in humans and is therefore highly prized as a vaccine carrier protein. The large and highly glycosylated KLH protein cannot be reproduced synthetically. It is available only as a purified biological product from the Keyhole Limpet Megathura crenulata.Keyhole limpet hemocyanin (KLH) is used extensively as a carrier protein in the production of for research, biotechnology and therapeutic applications. Haptens are substances with a low molecular weight such as peptides, small proteins and drug molecules that are generally not immunogenic and require the aid of a carrier protein to stimulate a response from the immune system in the form of antibody production.[2] KLH is the most widely employed carrier proteins for this purpose. KLH is an effective carrier protein for several reasons. Its large size and numerous epitopes generate a substantial immune response, and abundance of lysine residues for coupling haptens, allows a high hapten:carrier protein ratio increasing the likelihood of generating hapten-specific . In addition, because KLH is derived from the limpet, a gastropod, it is phylogenetically distant from mammalian proteins, thus reducing false positives in immunologically based research techniques in mammalian model organisms.KLH may also be a challenging molecule to work with because of its propensity to aggregate and precipitate. Aggregates remain immunogenic, but limit the ability to conjugate haptens and are difficult to manipulate in the laboratory.
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Purification method
Purified by Protein A.
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Storage
Water buffered solution containing 100ug/ml BSA, 50% glycerol and 0.09% sodium azide. Store at 4°C for 12 months.
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Excitation emission
590nm/617nm
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Synonyms
Keyhole limpet hemocyanin KLH
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Also known as
KLH Polyclonal Antibody
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Other name
Anti-KLH Polyclonal
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Advisory
Avoid freeze/thaw cycles as they may denaturate the polypeptide chains of the antibody, thus reducing its reactivity, specificity and sensitivity. For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube. Prior to use briefly centrifuge the vial to gather all the solution on the bottom.
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Properties
For facs or microscopy Alexa 1 conjugate.
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Conjugation
Alexa Fluor, ALEXA FLUOR® 594
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Conjugated
Alexa conjugate 1
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Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
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Group
Polyclonals and antibodies
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About
Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera.
