Anti-Adenovirus 5 E1A (Polyclonal), ALEXA Fluor 594

• Catalog number
  GENTObs-6136R-A594
• Price
  Please ask
• Size
  100 microliters

• Type
  Conjugated Primary Antibody
• Conjugated with
  ALEXA FLUOR® 594
• Host organism
  Rabbit (Oryctolagus cuniculus)
• Target Protein Peptide
  Adenovirus 5 E1A
• Specificity
  This antibody reacts specifically with Adenovirus 5 E1A
• Modification
  No modification has been applied to this antibody
• Modification site
  None
• Clonality
  Polyclonal Antibody
• Clone
  Polyclonal Antibodies
• Concentration
  1ug per 1ul
• Subcellular locations
  Nucleus
• Antigen Source
  KLH conjugated synthetic peptide derived from human Adenovirus 5 E1A
• Gene ID
  N/A
• Swiss Prot
  P03255
• Applications
  IF(IHC-P)
• Applications with corresponding dilutions
  IF(IHC-P)(1:50-200)
• Cross reactive species
  Virus
• Cross Reactive Species details
  Adenovirus5
• Background information
  The early region (E1) of the adenovirus genome, responsible for transforming activity, is localized within the left most 11% of the viral genome and consists of two transcriptional units E1A and E1B. E1A is sufficient for partial transformation and immortalization of primary cells. E1A gene products are necessary for normal levels of transcription of the other early regions of the adenovirus genome during productive infection and are able to either activate or repress the transcription of specific cellular genes. E1A forms specific complexes with cellular proteins including p105 causing inhibition of the cell cycle inducing arresting function of p105.
• Purification method
  Purified by Protein A.
• Storage
  Water buffered solution containing 100ug/ml BSA, 50% glycerol and 0.09% sodium azide. Store at 4°C for 12 months.
• Excitation emission
  590nm/617nm
• Synonyms
  Adenovirus early E1A protein; Control protein E1A; E1A; Early E1A protein; Human adenovirus type 5; Human adenovirus C; pfam02703; E1A_ADE05.
• Also known as
  Adenovirus 5 E1A Polyclonal Antibody
• Other name
  Anti-Adenovirus 5 E1A Polyclonal
• Advisory
  Avoid freeze/thaw cycles as they may denaturate the polypeptide chains of the antibody, thus reducing its reactivity, specificity and sensitivity. For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube. Prior to use briefly centrifuge the vial to gather all the solution on the bottom.
• Properties
  For facs or microscopy Alexa 1 conjugate.
• Conjugation
  Alexa Fluor, ALEXA FLUOR® 594
• Conjugated
  Alexa conjugate 1
• Description
  This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
• Group
  Polyclonals and antibodies
• About
  Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera.

• Gene target
  Adenovirus   E1A  
• Gene symbol
  ETV4
• Short name
  Anti-Adenovirus 5 E1A
• Technique
  Polyclonal, anti-, anti, antibody to, antibodies, Polyclonal antibodies (pAbs) are mostly rabbit or goat antibodies that are secreted by different B cells, whereas monoclonal antibodies come from a single N cell lineage. Pabs are a collection of immunoglobulin molecules that react against a specific antigen, each identifying a different epitope.
• Isotype
  Immunoglobulin G (IgG)
• Label
  ALEXA FLUOR 594
• Alternative name
  Adenovirus 5 E1A Polyclonal Antibody, ALEXA FLUOR® 594 Conjugated
• Alternative technique
  polyclonals, antibodies
• Virus
  adenovirus

Gene info

• Identity
  HGNC:3493
• Gene
  ETV4
• Long gene name
  ETS variant transcription factor 4
• Synonyms gene name
  • ets variant gene 4 (E1A enhancer-binding protein, E1AF)
  • ETS variant 4
• Synonyms
  • E1A-F
  • E1AF
  • PEA3
• Synonyms name
  • E1A enhancer binding protein
• GenBank acession
  • U18018
• Locus
  17q21.31
• Discovery year
  1995-03-27
• Entrez gene record
  2118
• Pubmed identfication
  • 8530053
  • 1547944
• RefSeq identity
  • NM_001986
• Classification
  • ETS transcription factor family
• VEGA ID
  OTTHUMG00000180884

MeSH Data

• Name
  Blotting, Western
• Concept
  Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
• Tree numbers
  • E05.196.401.143
  • E05.301.300.096
  • E05.478.566.320.200
  • E05.601.262
  • E05.601.470.320.200
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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