-
Type
Conjugated Primary Antibody
-
Conjugated with
ALEXA FLUOR 594
-
Host organism
Mouse (Mus musculus)
-
Target Protein Peptide
Endothelin-1
-
Specificity
Highly specific antibody against Endothelin-1
-
Modification
Not modified
-
Clonality
Monoclonal Antibodies
-
Clone
2C4
-
Concentration
1ug per 1ul
-
Antigen Source
KLH conjugated synthetic peptide derived from human Endothelin-1
-
Gene ID
1906
-
Applications
IF(IHC-P)
-
Applications with corresponding dilutions
IF(IHC-P)(1:50-200)
-
Cross Reactive species
Human (Homo sapiens), Rat (Rattus norvegicus)
-
Cross Reactive Species details
No significant cross reactivity is observed
-
Background information
Endothelins (ET) show potent constrictor activity in vascular and non-vascular smooth muscle. This family of 21-amino acid peptides exists in at least three isoforms - ET-1, ET-2, and ET-3, and is produced in endothelial and epithelial cells. ET's can mediate biological effects in cells and tissues, and have been shown to bind to an ET receptor in the lung, kidney, heart, and liver. Endothelin 1 is expressed in lung, placental stem villi vessels and in cultured placental vascular smooth muscle cells.
-
Purification method
Purified by Protein G.
-
Storage
Aqueous buffered solution containing 100ug/ml BSA, 50% glycerol and 0.09% sodium azide. Store at 4°C for 12 months.
-
Excitation emission
590nm/617nm
-
Synonyms
EDN 1; EDN1; EDN1 protein; Endothelin1; ET 1; ET1;
-
Alternative name 1
Endothelin-1(2C4) Monoclonal Antibody, ALEXA FLUOR® 594 Conjugated
-
Alternative name 2
Endothelin-1(2C4) Monoclonal anti-, ALEXA FLUOR 594 Conjugated
-
Advisory
Cycles of freezing and thawing should be avoided as such cycles may denature the peptide chains of the antibody and reduce its affinity, specificity and reactivity. For antibodies in a liquid form or reconstituted lyophilized antibodies - small amounts of the soultion could be captured on the cap or the walls of the container. Right before use you could briefly centrifuge the vial to collect all of the solution on the bottom.
-
Properties
For facs or microscopy Alexa 1 conjugate.
-
Conjugation
Alexa Fluor, ALEXA FLUOR® 594
-
Conjugated
Alexa conjugate 1
-
Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
-
About
Monoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes.