Rat Nerve Growth Factor (NGF) ELISA Kit (CLIA)
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Catalog numberEKU08827
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Price:
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Size96 Tests
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Estimated Turnaround Time6-11 Business days
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Target NameNerve Growth Factor
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Target s alterntive nameNGFB, Beta-NGF, HSAN5, NGF-B, Beta-Nerve Growth Factor
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Species ReactivityRattus norvegicus (Rat)
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Sample TypeSerum, Plasma, Tissue homogenates, Cell lysates, Cell culture supernates and other biological fluids
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Detection Range8.23-6000 pg/mL
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Sensitivity3.13 pg/mL
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PrecisionIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Nerve Growth Factor were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Nerve Growth Factor were tested on 3 different plates, 8 replicates in each plate.CV (%) = SD/meanX100;Intra-Assay: CV
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Detection MethodChemiluminescent
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Assay Time2 hours, 40minutes
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ApplicationFor research use only. Not for diagnostic procedures.
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Assay TypeDouble-antibody Sandwich
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Shipping ConditionsIce packs
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Storage TemperatureShort term: 4°C; Long term: see manual.
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Precaution of UseThe Stop Solution is acidic. Do not allow to contact skin or eyes.
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Shelf lifeUse Rat Nerve Growth Factor (NGF) ELISA Kit (CLIA) before 8 months
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SpecificityThis assay has high sensitivity and excellent specificity for detection of Nerve Growth Factor
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Cross ActivityNo significant cross-reactivity or interference between Nerve Growth Factor and analogues was observed.
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Test PrincipleNo significant cross-reactivity or interference between Nerve Growth Factor and analogues was observed.
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StabilityThe stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
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Quality SystemsThe kit is manufactured at ISO 9001 and ISO 13485 certified facilities.
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Research AreaCytokine, Neuro science
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PropertiesE05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays, E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays
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TestELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED
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DescriptionAplha, transcription related growth factors and stimulating factors or repressing nuclear factors are complex subunits of proteins involved in cell differentiation. Complex subunit associated factors are involved in hybridoma growth, Eosinohils, eritroid proliferation and derived from promotor binding stimulating subunits on the DNA binding complex. NFKB 105 subunit for example is a polypetide gene enhancer of genes in B cells.
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AboutRats are used to make rat monoclonal anti mouse antibodies. There are less rat- than mouse clones however. Rats genes from rodents of the genus Rattus norvegicus are often studied in vivo as a model of human genes in Sprague-Dawley or Wistar rats.
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Latin nameRattus norvegicus
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Gene target
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Gene symbolNGF, NGF-AS1
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Short nameNerve Growth Factor (NGF) ELISA Kit (CLIA)
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TechniqueELISA kit, ELISA, ELISAs Enzyme-linked immunosorbent assay 90320007 SNOMED code are used by the medical researcher for detection of human, mouse, … proteins are supplied in coated 96 well plates to be stored at +4°C. ELISA test kits can be sandwich ELISA. In sandwich ELISAS Enzyme-linked immunosorbent assay 9we use captor and detector antibodies. In single antibody ELISAs the antigen is coated and only a detector antibody is used. Traditional competition antigen ELISAs are coated with a captor antibody and a competitive antigen is labelled with the chromogen. In this case the ratio on your graph will be inverse proportional with the antigen present in your sample. A sign detected by the Colorimetric detection OD optical density absorbance at 450 nm between 0 and 4 with an ELISA reader for 450 nm absortion. Genprice Inc. the recognized authority tax ID 45-4304622 D-U-N-S number - 078440800. MeSH code E05 478 566 350 170 Enzyme-Linked Immunosorbent Assay. PROMT code 3841327 Immunoabsorbent assay tests. ELISA tests are enzyme linked immunoassays to detect human, mouse or other proteins in serum, plasma, urine or biological fluids. MeSH code E05 478 566 350 170 for Enzyme-Linked Immunosorbent Assay or ELISA kits. Also Enzyme-linked immunoassay, competitive have the SNOMED name 61461007 and the PROMT code for ELISA readers is 3841330. This is the desigation of other diagnostic analyzers and tests.
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HostRat, antigen assay ELISA kit 1 Kit (96 Wells)
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SpeciesRat, Rats
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Alternative nameRat Nerve Growth Factor (nerve growth factor (beta polypeptide)) Enzyme-linked immunosorbent assay reagent (CLIA)
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Alternative techniqueelisas, kits
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Alternative to gene targetv-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, transferase activity, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832
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Tissuenerve
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Gene info
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Identity
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Gene
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Long gene namenerve growth factor
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Synonyms gene
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Synonyms gene name
- nerve growth factor (beta polypeptide)
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Locus
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Discovery year2001-06-22
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Entrez gene record
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RefSeq identity
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Classification
- Neurotrophins
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VEGA ID
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Locus Specific Databases
Gene info
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Identity
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Gene
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Long gene nameNGF antisense RNA 1
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Synonyms
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Locus
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Discovery year2018-06-08
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Pubmed identfication
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Classification
- Antisense RNAs
MeSH Data
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Name
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ConceptScope note: An immunoassay utilizing an antibody labeled with an enzyme marker such as horseradish peroxidase. While either the enzyme or the antibody is bound to an immunosorbent substrate, they both retain their biologic activity; the change in enzyme activity as a result of the enzyme-antibody-antigen reaction is proportional to the concentration of the antigen and can be measured spectrophotometrically or with the naked eye. Many variations of the method have been developed.
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Tree numbers
- E05.478.566.350.170
- E05.478.566.380.360
- E05.478.583.400.170
- E05.601.470.350.170
- E05.601.470.380.360
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Qualifiersethics, mortality, psychology, trends, veterinary, history, classification, economics, instrumentation, methods, nursing, standards, adverse effects, statistics & numerical data