Human SYK (Spleen Tyrosine Kinase) ELISA Kit
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Catalog numberEKE61059
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Price:
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Size96 Tests
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Estimated Turnaround Time6-11 business days
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Target NameNA
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Target s alterntive nameSYK
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Species ReactivityHuman
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Sample TypeSerum, Plasma, Biological Fluids
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Detection Range0.63-40 ng/mL
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Sensitivity0.38 ng/mL
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PrecisionNA
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Detection MethodColorimetric
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Assay Time4.5 hours
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ApplicationFor research use only. Not for diagnostic procedures.
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Assay TypeSandwich
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Shipping ConditionsIce packs
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Storage TemperatureShort term: 4°C; Long term: see manual.
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Precaution of UseThe Stop Solution is acidic. Do not allow to contact skin or eyes.
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Shelf lifeUse Human SYK (Spleen Tyrosine Kinase) ELISA Kit before 6 months
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SpecificityNA
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Cross ActivityNo significant cross-reactivity or interference was observed.
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Test PrincipleThis ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human SYK . Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human SYK and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human SYK, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by adding Stop Solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human SYK. The concentration of Human SYK in samples can be calculated by comparing the OD of the samples to the standard curve.
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StabilityNA
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Quality SystemsThe kit is manufactured at ISO 9001 certified facilities.
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Research AreaCancer, Cardiovascular, Immunology, Signal transduction
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PropertiesE05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays, E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays, Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
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TestELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED
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Gene target
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Gene symbolSYK
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Short nameSYK (Spleen Tyrosine Kinase) ELISA Kit
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TechniqueELISA kit, ELISA, ELISAs Enzyme-linked immunosorbent assay 90320007 SNOMED code are used by the medical researcher for detection of human, mouse, … proteins are supplied in coated 96 well plates to be stored at +4°C. ELISA test kits can be sandwich ELISA. In sandwich ELISAS Enzyme-linked immunosorbent assay 9we use captor and detector antibodies. In single antibody ELISAs the antigen is coated and only a detector antibody is used. Traditional competition antigen ELISAs are coated with a captor antibody and a competitive antigen is labelled with the chromogen. In this case the ratio on your graph will be inverse proportional with the antigen present in your sample. A sign detected by the Colorimetric detection OD optical density absorbance at 450 nm between 0 and 4 with an ELISA reader for 450 nm absortion. Genprice Inc. the recognized authority tax ID 45-4304622 D-U-N-S number - 078440800. MeSH code E05 478 566 350 170 Enzyme-Linked Immunosorbent Assay. PROMT code 3841327 Immunoabsorbent assay tests. ELISA tests are enzyme linked immunoassays to detect human, mouse or other proteins in serum, plasma, urine or biological fluids. MeSH code E05 478 566 350 170 for Enzyme-Linked Immunosorbent Assay or ELISA kits. Also Enzyme-linked immunoassay, competitive have the SNOMED name 61461007 and the PROMT code for ELISA readers is 3841330. This is the desigation of other diagnostic analyzers and tests.
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Hostantigen assay ELISA kit 1 Kit (96 Wells)
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SpeciesHuman, Humans
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Alternative nameH. sapiens spleen tyrosine kinase (Spleen Tyrosine phosphorylation catalyst) Enzyme-linked immunosorbent assay reagent
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Alternative techniqueelisas, kits
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Alternative to gene targetv-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, transferase activity, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832
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Tissuespleen
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Gene info
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Identity
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Gene
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Long gene namespleen associated tyrosine kinase
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Synonyms gene name
- spleen tyrosine kinase
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GenBank acession
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Locus
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Discovery year1994-12-13
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Entrez gene record
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Pubmed identfication
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Classification
- Syk family tyrosine kinases
- SH2 domain containing
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VEGA ID
MeSH Data
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Name
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ConceptScope note: An immunoassay utilizing an antibody labeled with an enzyme marker such as horseradish peroxidase. While either the enzyme or the antibody is bound to an immunosorbent substrate, they both retain their biologic activity; the change in enzyme activity as a result of the enzyme-antibody-antigen reaction is proportional to the concentration of the antigen and can be measured spectrophotometrically or with the naked eye. Many variations of the method have been developed.
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Tree numbers
- E05.478.566.350.170
- E05.478.566.380.360
- E05.478.583.400.170
- E05.601.470.350.170
- E05.601.470.380.360
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Qualifiersethics, mortality, psychology, trends, veterinary, history, classification, economics, instrumentation, methods, nursing, standards, adverse effects, statistics & numerical data