Recombinant Escherichia coli Glutamate--cysteine ligase(gshA)
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Catalog number
RPC20137
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Price
Please ask
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Size
1 mg
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Verified reactivity
Escherichia coli (strain K12)
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Protein number
P0A6W9
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Gene number
gshA
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Other name
Gamma-ECS ; GCSGamma-glutamylcysteine synthetase
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Protein origin
E.coli
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Protein region
1-518aa
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Protein sequence
MIPDVSQALAWLEKHPQALKGIQRGLERETLRVNADGTLATTGHPEALGSALTHKWITTDFAEALLEFITPVDGDIEHMLTFMRDLHRYTARNMGDERMWPLSMPCYIAEGQDIELAQYGTSNTGRFKTLYREGLKNRYGALMQTISGVHYNFSLPMAFWQAKCGDISGADAKEKISAGYFRVIRNYYRFGWVIPYLFGASPAICSSFLQGKPTSLPFEKTECGMYYLPYATSLRLSDLGYTNKSQSNLGITFNDLYEYVAGLKQAIKTPSEEYAKIGIEKDGKRLQINSNVLQIENELYAPIRPKRVTRSGESPSDALLRGGIEYIEVRSLDINPFSPIGVDEQQVRFLDLFMVWCALADAPEMSSSELACTRVNWNRVILEGRKPGLTLGIGCETAQFPLPQVGKDLFRDLKRVAQTLDSINGGEAYQKVCDELVACFDNPDLTFSARILRSMIDTGIGGTGKAFAEAYRNLLREEPLEILREEDFVAEREASERRQQEMEAADTEPFAVWLEKHA
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Information about sequence
Full Length
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Expected molecular weight
74.23kDa
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Protein purity
≥ 91.2%
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Storage recommendation
Aliquot and store at -20°C. Minimize freezing and thawing.
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Use before
1 year
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Shipping requirements
Blue ice
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Estimated production time
7-11 business days
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Notes
For research use only. Not for diagnostic procedures.
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Gene
Cys or cysteines ChEMBL54943
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Source
Recombinants or rec. proteins
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Group
recombinants
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Gene target
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Short name
Recombinant Escherichia coli Glutamate--cysteine ligase(gshA)
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Technique
Recombinant, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. bioma advises they will be reconstituted in a buffer soluion or culture medium for cell culture.
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Host
Escherichia coli
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Label
N-terminal 6xHis-SUMO-tagged
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Alternative name
Rec. Escherichia coli Glutamate--cysteine ligase(gshA)
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Alternative technique
rec, escherichia
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MeSH Data
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Name
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Concept
Scope note:
A DNA amplification technique based upon the ligation of OLIGONUCLEOTIDE PROBES. The probes are designed to exactly match two adjacent sequences of a specific target DNA. The chain reaction is repeated in three steps in the presence of excess probe: (1) heat denaturation of double-stranded DNA, (2) annealing of probes to target DNA, and (3) joining of the probes by thermostable DNA ligase. After the reaction is repeated for 20-30 cycles the production of ligated probe is measured.
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Tree numbers
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Qualifiers
ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
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