Human Poly ADP Ribose Polymerase (PARP) CLIA Kit, 96 well plate
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Catalog numberEKU08862
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PricePlease ask
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Size5x96T
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Verified reactivityHomo sapiens (Human)
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Detection limits3.12-200 ng/mL
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Sensitivity limit0,11 ng/mL
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Samples to be analyzedTissue homogenates, cell lysates and other biological fluids
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Assay duration2 hours, 40min
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Verified applicationsCLIA
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Assay classSandwich
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ELISA detectionChemiluminescent
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Protein numberP09874
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Alternate protein numberPlease refer to SwissProt
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Gene number142
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Gene namePoly ADP Ribose Polymerase
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Alternate gene namePARP1; ADPRT; ADPRT1; PPOL; pADPRT-1; ADP-ribosyltransferase diphtheria toxin-like 1; NAD(+) ADP-ribosyltransferase 1
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Estimated production time7-11 business days
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Shipping requirementsBlue ice
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Storage recommendation-20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
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Use for8 months
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ELISA s specificityThis assay has high sensitivity and excellent specificity for detection of Poly ADP Ribose Polymerase (PARP). No significant cross-reactivity or interference between Poly ADP Ribose Polymerase (PARP) and analogues was observed.
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ELISA s cross reactivityThis assay doesn't seem to cross-react with other species. For more information about cross-reactivity please contact us.
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Assay principleThe microplate provided in this kit has been pre-coated with an antibody specific to Poly ADP Ribose Polymerase (PARP). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Poly ADP Ribose Polymerase (PARP). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Poly ADP Ribose Polymerase (PARP) level in the sample or standard.;
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ProtocolPlease see ELISA's datasheet, otherwise contact us
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Precision of the testIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Poly ADP Ribose Polymerase (PARP) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Poly ADP Ribose Polymerase (PARP) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV
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ELISA s stabilityThe stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
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QCThe Kit is manufactured at ISO 9001 and ISO 13485 certified facilities.
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Research main areaEnzyme & Kinase;
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WarningsDo not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
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NotesFor research use only. Not for diagnostic procedures.
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PropertiesHuman proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
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DescriptionA microtiter plate (spelled Microtiter is a registered trade name in the United States) or microplate or micro well plate or multiwell, is a flat plate with multiple "wells" used as small test tubes. The microplate has become a standard tool in analytical research and clinical diagnostic testing laboratories. A very common usage is in the enzyme-linked immunosorbent assay (ELISA), the basis of most modern medical diagnostic testing in humans and animals. A microplate typically has 6, 24, 96, 384 or 1536 sample wells arranged in a 23 rectangular matrix. Some microplates have even been manufactured with 3456 or 9600 wells, and an "array tape" product has been developed that provides a continuous strip of microplates embossed on a flexible plastic tape.
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Gene target
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Gene symbolWDTC1, ADPGK, TNKS2, TIPARP, PARP1, PARP12, TNKS
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Short namePoly ADP Ribose Polymerase (PARP) CLIA Kit, 96
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SpeciesHuman, Humans
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Alternative nameH. sapiens Poly ADP Ribose polymerizing enzyme (PARP) CLIA reagent, 96 well plate
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Alternative techniquekits
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Alternative to gene targetv-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, transferase activity, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832
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Gene info
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Identity
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Gene
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Long gene nameWD and tetratricopeptide repeats 1
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year2003-12-22
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- WD repeat domain containing
- Tetratricopeptide repeat domain containing
- DDB1 and CUL4 associated factors
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameADP dependent glucokinase
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Synonyms
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GenBank acession
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Locus
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Discovery year2004-09-29
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nametankyrase 2
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Synonyms gene name
- tankyrase, TRF1-interacting ankyrin-related ADP-ribose polymerase 2
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year2001-05-10
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Entrez gene record
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RefSeq identity
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Classification
- Poly(ADP-ribose) polymerases
- Sterile alpha motif domain containing
- Ankyrin repeat domain containing
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameTCDD inducible poly(ADP-ribose) polymerase
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Synonyms gene name
- TCDD-inducible poly(ADP-ribose) polymerase
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Synonyms
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GenBank acession
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Locus
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Discovery year2003-12-02
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Poly(ADP-ribose) polymerases
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VEGA ID
Gene info
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Identity
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Gene
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Long gene namepoly(ADP-ribose) polymerase 1
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Synonyms gene
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Synonyms gene name
- ADP-ribosyltransferase (NAD+; poly (ADP-ribose) polymerase)
- poly (ADP-ribose) polymerase family, member 1
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Synonyms
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GenBank acession
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Locus
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Discovery year1986-01-01
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Zinc fingers PARP-type
- Zinc fingers
- Poly(ADP-ribose) polymerases
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VEGA ID
Gene info
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Identity
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Gene
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Long gene namepoly(ADP-ribose) polymerase family member 12
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Synonyms gene
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Synonyms gene name
- zinc finger CCCH-type domain containing 1
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Synonyms
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GenBank acession
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Locus
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Discovery year2003-12-10
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Poly(ADP-ribose) polymerases
- Zinc fingers CCCH-type
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nametankyrase
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Synonyms gene name
- tankyrase, TRF1-interacting ankyrin-related ADP-ribose polymerase
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year1999-01-08
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Poly(ADP-ribose) polymerases
- Sterile alpha motif domain containing
- Ankyrin repeat domain containing
- MicroRNA protein coding host genes
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VEGA ID