BIOKÉ

BIOKÉ is a life sciences company based in Europe, specializing in innovative research solutions for molecular biology, genomics, proteomics, and cell biology. The company provides high-quality reagents, instruments, and software to support academic, pharmaceutical, and biotech research.

Key Areas of Expertise:

Genomics & Next-Generation Sequencing (NGS)
Molecular & Cell Biology Research
Proteomics & Immunology
Epigenetics & Gene Regulation
Automation & High-Throughput Screening

Industries Served:

Academic and government research institutions
Biotech and pharmaceutical companies
Clinical and diagnostic laboratories

Browse BIOKÉ Products

(+) -JQ1

SKU: CST 84566S|Size: 5 mg

0.5 M EDTA, pH 8.0

SKU: CST 7011V|Size: 5 mL

1 kb Plus DNA Ladder

SKU: NEB N3200L|Size: 1000 Gel Lanes

1 kb Plus DNA Ladder for Safe Stains

SKU: NEB N0559S|Size: 250 Gel Lanes

1" Half wave extender, w/replaceable tip

SKU: Q408HWT|Size: 1 Piece

1" High gain probe w/ replaceble tip

SKU: Q4311|Size: 1 Piece

1" High gain probe w/ solid tip

SKU: Q4310|Size: 1 Piece

1" probe w/ replaceable tip

SKU: Q4210|Size: 1 Piece

1" replacement tip

SKU: Q4408|Size: 1 Piece

1" solid probe

SKU: Q4209|Size: 1 Piece

1/16" microtip (0.2 - 5ml)

SKU: Q4417|Size: 1 Piece

1/2" Half wave extender, w/replaceable tip

SKU: Q406HWT|Size: 1 Piece

1/2" Half wave extender, w/solid tip

SKU: Q406HW|Size: 1 Piece

1/2" probe w/ replaceable tip

SKU: Q4220|Size: 1 Piece

1/2" replaceable tip probes (qty. 2 probes - matched set for dual horn)

SKU: Q4220M|Size: 1 Piece

1/2" replacement tip

SKU: Q4406|Size: 1 Piece

1/2" solid probe

SKU: Q4219|Size: 1 Piece

1/4" microtip (5 - 50ml)

SKU: Q4420|Size: 1 Piece

1/8" microtip (1 - 15ml)

SKU: Q4418|Size: 1 Piece

10% SDS Solution

SKU: CST 20533S|Size: 50 mL

10058-F4

SKU: CST 66672S|Size: 5 mg

100X Spermidine

SKU: CST 27287S|Size: 1.3 mL

10X High Salt Wash Buffer (CUT&Tag)

SKU: CST 18878S|Size: 3 mL

10X Wash Buffer (CUT&RUN, CUT&Tag)

SKU: CST 31415S|Size: 15 mL

10X Wash Buffer, Phosphate Buffered Saline (PBS)

SKU: CST 12528S|Size: 200 mL

12 Channel Reservoir Plate, 21ml channels, pyramid bottom, clear PP, 25 plates per case

SKU: 4TI-0131|Size: 25/Box

12 Channel Reservoir Plate, 290ml open format, 12 channel pyramid bottom, clear PP, 25 plates per case

SKU: 4TI-0133|Size: 25 Plates (5 Plates/Pack, 5 Packs/Case)

12-Tube Magnetic Separation Rack

SKU: CST 14654S|Size: 1 Each

125 watt Sonicator, 220V, includes 1/8" probe

SKU: Q125-220|Size: 1 Piece

125 watt Sonicator, 220V, includes 1/8" probe and sound enclosure

SKU: Q125E-220|Size: 1 Piece

125 watt Sonicator, 220V, without a probe

SKU: Q125A-220|Size: 1 Piece

14-3-3 (pan) (E9S9M) Rabbit Monoclonal Antibody

SKU: CST 95422S|Size: 100 µL

14-3-3 (pan) Antibody

SKU: CST 8312S|Size: 100 µL

14-3-3 Family Antibody Sampler Kit

SKU: CST 9769T|Size: 1 Kit

14-3-3 β/α Antibody

SKU: CST 9636S|Size: 100 µL

14-3-3 γ (D15B7) Rabbit Monoclonal Antibody

SKU: CST 5522S|Size: 100 µL

14-3-3 ε Antibody

SKU: CST 9635S|Size: 100 µL

14-3-3 ζ/δ (D7H5) Rabbit Monoclonal Antibody

SKU: CST 7413S|Size: 100 µL

14-3-3 η (D23B7) Rabbit Monoclonal Antibody

SKU: CST 5521S|Size: 100 µL

14-3-3 η Antibody

SKU: CST 9640S|Size: 100 µL

14-3-3 τ Antibody

SKU: CST 9638S|Size: 100 µL

15-PGDH/HPGD (E9H7W) Rabbit Monoclonal Antibody

SKU: CST 94019T|Size: 20 µL

15mL X 50 mL Combo Magnetic Separation Rack 3X3

SKU: MBG MBMS-31550|Size: 1 Item

15ml Cytometer tube holder for MARS

SKU: APC TH15|Size: 1 Piece

16 Tip horn

SKU: Q4699|Size: 1 Piece

16 Tube Holder

SKU: Q4100|Size: 1 Piece

16 Well Non-Skirted PCR Plate Segment, blue PP, high profile, 300 segments per case

SKU: 4TI-0750/16/B|Size: 300 Segments

16 Well Non-Skirted PCR Plate Segment, clear PP, high profile, 300 segments per case

SKU: 4TI-0750/16|Size: 300 Segments

16 Well Non-Skirted PCR Plate Segment, green PP, high profile, 300 segments per case

SKU: 4TI-0750/16/G|Size: 300 Segments

16 Well Non-Skirted PCR Plate Segment, purple PP, high profile, 300 segments per case

SKU: 4TI-0750/16/P|Size: 300 Segments

16 Well Non-Skirted PCR Plate Segment, red PP, high profile, 300 segments per case

SKU: 4TI-0750/16/R|Size: 300 Segments

16 Well Non-Skirted PCR Plate Segment, yellow PP, high profile, 300 segments per case

SKU: 4TI-0750/16/Y|Size: 300 Segments

16% Formaldehyde, Methanol-Free

SKU: CST 12606S|Size: 50 mL

17-AAG

SKU: CST 8132S|Size: 500 µg

2.5'' Horn for Q431C2 Cuphorn

SKU: Q4623|Size: 1 Piece

20 mM Citrate pH 3.0 (Sterile)

SKU: CST 9871L|Size: 50 mL

200 mM HEPES, pH 8.0

SKU: CST 44686S|Size: 60 mL

2000 watt Sonicator system w/ flocell

SKU: Q2000F|Size: 1 Piece

2000 watt Sonicator system, 220V

SKU: Q2000|Size: 1 System

2000 watt system with 4705 Flocell (incl. probe, booster, enclosure, stand, 220V

SKU: Q2000F1E-220|Size: 1 Piece

2000 watt system with 4777 probe, 220V

SKU: Q2000-220|Size: 1 System

20X LumiGLO® Reagent and 20X Peroxide

SKU: CST 7003S|Size: 50 mL

24 Tube holder for 1.5ml tubes

SKU: Q4259|Size: 1 Piece

24 Well Assay Plate, 1.88ml round wells, flat base, black PS, cut corner A1,100 plates per case

SKU: 4TI-0262|Size: 100 Plates

24 Well Non-Skirted PCR Plate Segment, blue PP, high profile, 200 segments per case

SKU: 4TI-0750/24/B|Size: 200 Segments

24 Well Non-Skirted PCR Plate Segment, clear PP, high profile, 200 segments per case

SKU: 4TI-0750/24|Size: 200 Segments

24 Well Non-Skirted PCR Plate Segment, green PP, high profile, 200 segments per case

SKU: 4TI-0750/24/G|Size: 200 Segments

24 Well Non-Skirted PCR Plate Segment, purple PP, high profile, 200 segments per case

SKU: 4TI-0750/24/P|Size: 200 Segments

24 Well Non-Skirted PCR Plate Segment, red PP, high profile, 200 segments per case

SKU: 4TI-0750/24/R|Size: 200 Segments

24 Well Non-Skirted PCR Plate Segment, yellow PP, high profile, 200 segments per case

SKU: 4TI-0750/24/Y|Size: 200 Segments

24-Square-well Block with silicone lid (lysis plate)

SKU: MN 740679.4|Size: 4 Plates

2B4/SLAMF4/CD244 (D5J9D) Rabbit Monoclonal Antibody

SKU: CST 54560S|Size: 100 µL

2’,3’-cGAMP (sodium salt)

SKU: CST 35573S|Size: 500 µg

3'-Desthiobiotin-GTP

SKU: NEB N0761S|Size: 0.5 µmol

3/4" Half wave extender, w/replaceable tip

SKU: Q407HWT|Size: 1 Piece

3/4" High gain probe w/ replaceble tip

SKU: Q4305|Size: 1 Piece

3/4" High gain probe w/ solid tip

SKU: Q4306|Size: 1 Piece

3/4" probe w/ replaceable tip

SKU: Q4207|Size: 1 Piece

3/4" replacement tip

SKU: Q4407|Size: 1 Piece

3/4" solid probes (qty. 2 probes - matched set for dual horn)

SKU: Q4208M|Size: 1 Piece

32 Well Non-Skirted PCR Plate Segment, blue PP, high profile, 150 segments per case

SKU: 4TI-0750/32/B|Size: 150 Segments

32 Well Non-Skirted PCR Plate Segment, clear PP, high profile, 150 segments per case

SKU: 4TI-0750/32|Size: 150 Segments

32 Well Non-Skirted PCR Plate Segment, green PP, high profile, 150 segments per case

SKU: 4TI-0750/32/G|Size: 150 Segments

32 Well Non-Skirted PCR Plate Segment, purple PP, high profile, 150 segments per case

SKU: 4TI-0750/32/P|Size: 150 Segments

32 Well Non-Skirted PCR Plate Segment, red PP, high profile, 150 segments per case

SKU: 4TI-0750/32/R|Size: 150 Segments

32 Well Non-Skirted PCR Plate Segment, yellow PP, high profile, 150 segments per case

SKU: 4TI-0750/32/Y|Size: 150 Segments

384 Square Deep Well Storage Microplate, 190ul square wells, V-shaped bottom, clear PP, 100 plates per case

SKU: 4TI-0147|Size: 100 Plates

384 Square Well Sealing Cap Mat, clear silicone, for use with square 384 well microplates and deep well storage microplates, 50 mats per case

SKU: 4TI-0139|Size: 50 Mats

384 Well Assay Plate, 0.12ml rounded square wells, flat base, black PS, cut corner A1/P1,100 plates per case

SKU: 4TI-0264|Size: 100 Plates

384 Well Assay Plate, 0.12ml rounded square wells, flat base, clear PS, cut corner A1/P1,100 plates per case

SKU: 4TI-0254|Size: 100 Plates

384 Well Assay Plate, 0.12ml rounded square wells, flat base, white PS, cut corner A1/P1,100 plates per case

SKU: 4TI-0274|Size: 100 Plates

384 Well Skirted PCR Plate, Roche style, white PP, cut corner A24/P24,50 plates per case

SKU: 4TI-1381|Size: 50 Plates

384 Well Skirted PCR Plate, clear PP, cut corner A24,50 plates per case

SKU: 4TI-1384|Size: 50 Plates

384 Well Skirted PCR Plate, frosted PP, cut corner A24,50 plates per case

SKU: 4TI-1387|Size: 50 Plates

384 Well Skirted PCR Plate, white PP, cut corner A24,50 plates per case

SKU: 4TI-1385|Size: 50 Plates

3X DYKDDDDK Tag (E2T2J) Mouse Monoclonal Antibody

SKU: CST 33542T|Size: 20 µL

3X DYKDDDDK Tag (E2T2J) Mouse Monoclonal Antibody (HRP Conjugate)

SKU: CST 69881S|Size: 100 µL

3X DYKDDDDK Tag (E7C5T) Rabbit Monoclonal Antibody

SKU: CST 87537S|Size: 100 µL

3X DYKDDDDK Tag (E7C5T) Rabbit Monoclonal Antibody (HRP Conjugate)

SKU: CST 74869S|Size: 100 µL

3´-O-Me-m7G (5') ppp (5') G RNA Cap Structure Analog (ARCA)

SKU: NEB S1411S|Size: 1 µmol

Recent Breakthroughs

Magnetic-aminopropyltriethoxysilane-sulfanilamide: a new functional sorbent for selective preconcentration of sulfonylurea antidiabetic drugs in biological samples.

Ulusoy S, İrem Altunbaş Ş, Gürbüzer A, Polat Ü, İbrahim Ulusoy H.

Analyst

PMID:41804642

Free PMC article

A highly selective and sensitive analytical strategy was established for the trace quantification of two oral antidiabetic drugs, gliclazide (GLZ) and glimepiride (GLM). The procedure integrates magnetic solid-phase extraction (MSPE) with high-performance liquid chromatography coupled to diode array detection (HPLC-DAD). In this approach, a newly engineered magnetic-aminopropyltriethoxysilane-sulfanilamide (Fe 3 O 4 @APTES-sulfanilamide) was synthesized and utilized as an innovative adsorbent. Sulfanilamide as a functional molecule on the surface has been used for the first time in this study. The hybrid structure, consisting of a Fe 3 O 4 magnetic core and a sulfanilamide-functionalized silane shell, provides strong binding affinity toward sulfonylurea compounds through synergistic hydrogen bonding and π-π interactions. Optimization studies were carried out to achieve the best extraction efficiency by adjusting experimental variables such as solution pH, adsorption and desorption times, and solvent composition. Under the final working conditions (pH 6.0; desorption with an acetonitrile-methanol mixture), both target analytes were effectively preconcentrated prior to chromatographic determination. Separation was accomplished using an isocratic elution system containing 10% methanol, 40% phosphate buffer (pH 3.0), and 50% acetonitrile, with UV detection at 219 and 256 nm. The developed protocol demonstrated excellent linearity, remarkable enrichment factors, and very low detection limits. Precision studies yielded RSD values below 3.5% ( n = 3, 100 ng mL -1 ). The reliability of the proposed method was further validated by its successful application to both synthetic and human urine samples, giving satisfactory recovery results. This newly designed Fe 3 O 4 @APTES-sulfanilamide -based MSPE coupled with HPLC-DAD provides a robust, time-efficient, and eco-friendly platform for the determination of trace oral antidiabetic drugs in complex biological matrices.

Measurements of plasma melatonin by rapid lC-mS/mS enables insight into diurnal variation in healthy humans.

Albrethsen J, Andreasen JK, Anton J, Sennels HP, Jørgensen HL, Hannibal J, Hermann D, Wewer Albrechtsen NJ.

Scand J Clin Lab Invest

PMID:42008634

Free PMC article

Measurement of plasma melatonin is important in studies of sleep disorders and neurodegenerative diseases and is also increasingly associated with cardiometabolic diseases and cancer. There is currently an increase in the usage of melatonin in the population. However, commercially available and reliable melatonin assays are lacking. Here we present a rapid and simple LC-MS/MS-based method for plasma melatonin in a clinical laboratory. The method demonstrates a limit of detection of 5 ng/L and inter-assay imprecision p p < 0.03) but demonstrated a substantial bias. We conclude that our simple LC-MS/MS method can detect biologically relevant changes in plasma melatonin and may enable insight into circadian biology in humans. We propose that the observed differences between melatonin assays could be clarified by the distribution of reference plasma melatonin material and that additional studies are needed to fully understand the minimum circulating levels of melatonin in the daytime.

A Targeted LC-MS/MS Assay of a Health Surveillance Panel and Its Application to Chronic Kidney Disease.

Fu Q, Johnson CWC, Inker LA, Van Eyk JE, CRIC Study Investigators.

J Proteome Res

PMID:42007652

Free PMC article

Robust and reproducible assays capable of specific and quantitative monitoring of multiple biologically important proteins, among the thousands of human plasma proteins, can be used to represent the overall health of an individual and distinguish health versus disease. In this study, we established an LC-MS assay to monitor a Health Surveillance Panel (HSP), comprising 60 circulating plasma proteins selected based on their biological functions and/or disease associations. Plasma samples were prepared for proteomic analysis in an automated process and analyzed using an optimized, scheduled LC-MRM assay composed of 60 endogenous proteins monitored by 364 transitions from 117 proteotypic peptides, along with their stable isotopically labeled standard peptides. For each proteotypic peptide, a quantifier ion and at least two qualifier ions were selected based on consistent peak area ratios, a linear response for the quantifier ion, and a low limit of quantification. As proof of concept, we evaluated the performance of our HSP assay in a case-control study of progressive chronic kidney disease (CKD). Reduced plasma concentrations of alpha-2-antiplasmin, antithrombin-III, and immunoglobulin heavy constant alpha 1 correlated with CKD, with p -values <0.05. These results demonstrate that CKD-associated differences can be detected with a multiplexed HSP assay panel.

Development and validation of ratio spectra manipulation methods for the determination of etoricoxib and tramadol in binary mixtures.

Al Kamaly O, Sayedahmed M, Abdelzaher AM.

Sci Rep

PMID:42010288

Free PMC article

Two simple and selective spectrophotometric methods, ratio difference and derivative ratio, were developed and validated for the simultaneous determination of etoricoxib and tramadol in binary mixtures and pharmaceutical formulations. The methods effectively resolved the sever spectral overlap of both drugs without prior separation. In the ratio difference method, amplitude differences between 294 and 239 nm for etoricoxib and 218 and 272 nm for tramadol showed excellent linearity. In the derivative ratio method, derivative amplitudes measured at 287 nm etoricoxib, and 229 nm tramadol provided high selectivity and sensitivity. The linear ranges were 2-23 µg/mL for etoricoxib and 3-40 µg/mL for tramadol, with correlation coefficients above 0.999. Both methods achieved recoveries between 98.78 and 101.09% and precision values (%RSD) below 2%. Statistical comparison with a reported HPLC method showed no significant difference. The proposed methods are accurate, cost-effective, and supported by AGREE-based greenness assessment, making them suitable for routine quality control of etoricoxib and tramadol formulations.

Molecular strategies for heterocyclic frameworks in antidiabetic drug discovery: a vision from 2020-2024.

Tahlan S, Singh S, Dey H, Kaira M, Pandey KC.

Future Med Chem

PMID:42003351

Free PMC article

Diabetes mellitus represents a global health crisis requiring innovative therapeutic strategies beyond traditional treatments. This comprehensive review analyzes heterocyclic frameworks developed between 2020-2024 for antidiabetic drug discovery, highlighting structure-activity relationships (SAR), molecular docking insights, and therapeutic mechanisms. Key scaffold classes emerged as potent antidiabetic agents, with benzimidazoles and triazoles demonstrating dual α-amylase/α-glucosidase inhibition (IC 50 values 1.20-22.46 µg/mL), thiazolidinediones and quinazolines showing PPAR-γ agonism with improved insulin sensitivity and reduced cardiovascular risks, DPP-4 inhibitory scaffolds (pyrrolidines, pyrimidines) achieving IC 50 values as low as 0.021 µM, and SGLT2-targeting heterocycles exhibiting glucose-lowering effects with cardio-renal protection. Major findings revealed that electron-donating groups (methoxy, hydroxyl) consistently enhanced binding affinity across multiple targets, halogen substitutions (fluoro, chloro, bromo) improved metabolic stability and selectivity, hybrid molecules combining multiple pharmacophores achieved superior multi-target effects, and natural product-derived heterocycles (flavonoids, coumarins, alkaloids) showed IC 50 values 10-100× better than acarbose. Emerging frontiers include multi-agonist therapies (GLP-1/GIP, dual SGLT1/SGLT2 inhibitors), glucokinase activators for insulin-independent glucose control, microbiome-targeting agents, and AI-driven rational drug design integrating SAR, docking, and ADMET prediction. This review provides a strategic framework for developing safer, more selective antidiabetic agents through systematic exploitation of heterocyclic chemistry, advancing toward personalized diabetes management.