Rabbit Polyclonal Ig Anti-Borrelia
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Catalog number
BOR001
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Price
Please ask
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Size
200 µg
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Availability
Please contact us to check the availability
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Immunogen
Cell preparation of Borrelia burgdorferi
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Specificity
Borrelia burgdorferi, cross-reactivity with Borrelia hermsii and Borrelia parkerii as well as Treponema pallidum. In Western blotting bands are seen at 83kDa, 41kDa, 34kDa, 31kDa. Additional lower MW bands may also be observed.
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Species reactivity
Borrelia, (Treponema)
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Product presentation
Purified IgG in PBS pH7.2, contains sodium azide (0,09%)**
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Product category
Primary Antibody
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Antibody s raised in
Rabbit
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Antibody s isotype
Ig
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Antibody s clone
polyclonal
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Product is supplied as
liquid concentrate
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Recommended concentration for use
WB: 1 µg/ml, IHC: 5 µg/ml
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Protein concentration
1 mg/ml
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Tested applications
IHC(P), WB, IFA, ELISA
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Recommended secondary reagent
Anti-Rabbit IgG:Biotin-conjugate (Art. No. ZU101) in combination with Streptavidin:HRPO (Art. No. ZU054) or Streptavidin:AP (Art. No. ZU051)
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Storage method
Store at 2-8°C
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UniProt number
Go to UniProt/SwissProt
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Litterature
1. Buechner S A , Winkelmann R K, Lautenschlager S ,Gilli L , Rufli T (1993) Localized scleroderma associated with Borrelia burgdorferi infection. Clinical, histologic, and immunohistochemical observations Journal of the American Academy of Dermatology. 29(2 Pt 1):190-6. 2. Quatresooz P., Piérard G. E. (2009) Skin Homing of Treponema pallidum in Early Syphilis: An Immunohistochemical Study Applied Immunohistochemistry & Molecular Morphology: Volume 17 - Issue 1 - pp 47-50 3. Arrese Estrada J; Mélotte P; Hermanns J F; Piérard G E (1991) Immunohistochemistry of Borrelia type spirochetes Annales de dermatologie et de vénéréologie 118(4):277-9.
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Tips
*Gentaur's antibodies are intended for in vitro research use only. They must not be used for clinical diagnostics and not for in vivo experiments in humans or animals. ** The preservative sodium azide is known to be poisonous and potentially hazardous to health. It should be handled only by trained staff. Despite of the product's low azide concentration it must be handled with care. Dispose according to regional rules!
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Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
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Group
Polyclonals and antibodies
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About
Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera. Rabbits are used for polyclonal antibody production by bio logo. Rabbit antibodies are very stable and can be stored for several days at room temperature. bio logo adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
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Latin name
Oryctolagus cuniculus
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Gene target
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Short name
Research
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Technique
Polyclonal, Rabbit, anti-, anti, antibody to, antibodies, Polyclonal antibodies (pAbs) are mostly rabbit or goat antibodies that are secreted by different B cells, whereas monoclonal antibodies come from a single N cell lineage. Pabs are a collection of immunoglobulin molecules that react against a specific antigen, each identifying a different epitope.
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Host
Rabbit, Rabbits
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Alternative name
Borrelia
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Alternative technique
polyclonals, rabbit-anti, antibodies
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Disease
borrelia
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MeSH Data
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Name
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Concept
Scope note:
Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
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Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
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Qualifiers
ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
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