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Availability
Please contact us to check the availability
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Immunogen
Immunodiffusion precipitate of ovarian cystic mucins with NS19-9-antibody
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Specificity
Sialyl Lewis a antigen (Cancer Antigen CA 19-9)
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Species reactivity
Human
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Product presentation
Antibody solution in stabilizing phosphate buffer pH 7.3. Contains 0.09 % sodium azide**. The volume is sufficient for at least 100 immunohistochemical tests (100 µl working solution / test). Use appropriate antibody diluent e.g. Gentaur Art .No. PU002.
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Product category
Primary Antibody
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Antibody s raised in
Mouse
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Antibody s isotype
IgM
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Antibody s clone
121 SLE
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Product is supplied as
liquid concentrate
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Recommended concentration for use
1:10 - 1:50
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Protein concentration
50 µg/ml
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Positive control
Colon carcinoma
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Pretreatment
In paraffin section no pre-treatment is required, however heat induced antigen retrieval may improve the staining. Each laboratory should select it's own optimum staining and pre-treatment conditions.
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Tested applications
ELISA, IRMA, IHC (cryostat and paraffin sections)
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Recommended secondary reagent
As secondary reagents we recommend the use of biotinylated anti-mouse IgM antibody (Art. No. BA-2020) in combination with streptavidin conjugates or ABC systems.
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Storage method
Store at 2-8°C
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UniProt number
Go to UniProt/SwissProt
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Litterature
1. Gatalica Z., and Miettienen M. (1994) Distribution of carcinoma antigens CA 19-9 and CA 15-3: an immunohistochemical study of 400 tumors. Appl. Immunohistochem. 2(3); 205ff. 2. Tomazic A., and Pegan V. (2000) Preoperative staging of periampullar cancer with US, CT, EUS and CA 19-9. Hepatogastroenterol. 47(34); 1135-1137. 3. Tabata T., Takeshima N., Tanaka N., Hirai Y., and Hasumi K. (2000) Clinical value of tumor markers for early detection of recurrence in patients with cervical adenocarcinoma and adenosquamous carcinoma. Tumour Biol. 21(6); 375-380. 4. Dalgleish A.G. (2000) Tumour markers in malignancies. CA19.9 is useful in several cancers. B.M.J. 321(7257); 380
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Tips
*Gentaur's antibodies are intended for in vitro research use only. They must not be used for clinical diagnostics and not for in vivo experiments in humans or animals. ** The preservative sodium azide is known to be poisonous and potentially hazardous to health. It should be handled only by trained staff. Despite of the product's low azide concentration it must be handled with care. Dispose according to regional rules!
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Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
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Properties
Immunoglobulin M, or IgM for short, is a basic antibody that is produced by B cells. IgM is by far the physically largest antibody in the human circulatory system. It is the first antibody to appear in response to initial exposure to an antigen
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About
Monoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes.
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Test
Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
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Latin name
Mus musculus
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Gene target
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Gene symbol
PIRC99, PIRC102, PIRC100, PIRC98, PIRC101
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Short name
Research
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Technique
Mouse monoclonal, anti-, Mouse, anti, IgM, antibody to, antibodies, IgMs, Monoclonals or monoclonal antibodies, mouses, mouse monoclonals
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Host
mouse monoclonal, Murine monoclonal antibodies or Mabs are often conjugated and the isotype is IgG.
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Isotype
IgM, IgM
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Species
Mouse, Mouses
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Alternative name
CA 19-9, Sialyl-Lewis a
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Alternative technique
murine, antibodies, immunoglobulin-m
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