anti-HIV-1 Reverse Transcriptase antibody, rabbit serum, WB, Dot, IP, ELISA
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Catalog number
65-001
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Price
Please ask
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Size
100ul
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Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use. Reverse transcription primers are used in PCR but in vivo reverse transcription begins when the viral particle that enters the cytoplasm of a target cell with its reverse transcriptase. The viral RNA genome enters the cytoplasm as part of a nucleoprotein complex that has not been well characterized. The process of reverse transcription generates, in the cytoplasm, a linear DNA via an intricate series of steps. This DNA is collinear with its RNA template, but it contains terminal duplications known as the long terminal repeats (LTRs) that are not present in viral RNA . Extant models for reverse transcription propose that two specialized template switches known as strand-transfer reactions or “jumps” are required to generate the LTRs. Azide Free Serum for cell culture FBS fetal bovine serum. Filtered serum for sterility. The polyclonal serum contains no red or white blood cells or clotting factors; it is the blood plasma not including the fibrinogens. Serum includes all proteins and antibodies not used in blood clotting (coagulation) and all the electrolytes, antibodies, antigens, hormones, and any exogenous substances like drugs and microorganisms. Plasmas on request.
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Properties
If you buy Antibodies supplied by AS ONE INTERNATIONAL they should be stored frozen at - 24°C for long term storage and for short term at + 5°C. E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays
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Test
Dot blots are simpler than Western blot since no electrophoresis needed. It is advised to use PVDF or Nitrocellulose to deposit the protein samples that will be incubated with the blotting serum before ECL or colorimetric detection. ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED
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Gene
The human immunodeficiency virus (HIV) is a lentivirus (a subgroup of retrovirus) that causes HIV infection and over time acquired immunodeficiency syndrome(AIDS). AIDS is a condition in humans in which progressive failure of the immune system allows life-threatening opportunistic infections and cancers to thrive. Without treatment, average survival time after infection with HIV is estimated to be 9 to 11 years, depending on the HIV subtype. Infection with HIV occurs by the transfer of blood, semen, vaginal fluid, pre-ejaculate, or breast milk. Within these bodily fluids, HIV is present as both free virus particles and virus within infected immune cells. recombinant HIV 1 and 2 gag gene proteins p24, p17, p55 immunodominant epitopes and envelope glycoproteins, gp120 are used for production of diagnostic detection antibodies.
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About
Rabbits are used for polyclonal antibody production by AS ONE INTERNATIONAL. Rabbit antibodies are very stable and can be stored for several days at room temperature. AS ONE INTERNATIONAL adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long. Serum should be stored at +5°C
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Latin name
Oryctolagus cuniculus
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Goup
reverse transcription
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Group
Sera
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French translation
anticorps
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Gene target
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Gene symbol
HIVEP2, IGHVII-1-1, MIR1-1, TRUND-NNN8-1, TRUND-NNN7-1, IGKV1OR2-1, MIR101-1, MIR1289-1, MIR16-1
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Short name
anti-HIV-1 Reverse Transcriptase antibody, rabbit serum, , Dot, IP, ELISA
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Technique
Antibody, reverse, Rabbit, anti-, ELISA, serum, anti, dot, Dot, IP, WB, antibody to, antibodies, antibodies against human proteins, antibodies for, cicular dots, ELISAs Enzyme-linked immunosorbent assay 90320007 SNOMED code are used by the medical researcher for detection of human, mouse, … proteins are supplied in coated 96 well plates to be stored at +4°C. ELISA test kits can be sandwich ELISA. In sandwich ELISAS Enzyme-linked immunosorbent assay 9we use captor and detector antibodies. In single antibody ELISAs the antigen is coated and only a detector antibody is used. Traditional competition antigen ELISAs are coated with a captor antibody and a competitive antigen is labelled with the chromogen. In this case the ratio on your graph will be inverse proportional with the antigen present in your sample. A sign detected by the Colorimetric detection OD optical density absorbance at 450 nm between 0 and 4 with an ELISA reader for 450 nm absortion. Genprice Inc. the recognized authority tax ID 45-4304622 D-U-N-S number - 078440800. MeSH code E05 478 566 350 170 Enzyme-Linked Immunosorbent Assay. PROMT code 3841327 Immunoabsorbent assay tests. Immunoprecipitation, Western Blot on a membrane
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Host
Rabbit, antigen assay ELISA kit 1 Kit (96 Wells), Rabbits
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Alternative name
Antibody toHIV-1 Reverse Transcriptase (antibody to-), production species: rabbit blood serum, Western Blot, Dot, IP, Enzyme-linked immunosorbent assay
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Alternative technique
antibodies, transctription, rabbit-anti, sera, elisas, blots
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Tissue
serum
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Virus
hiv
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Gene info
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Identity
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Gene
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Long gene name
HIVEP zinc finger 2
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Synonyms gene name
- human immunodeficiency virus type I enhancer-binding protein 2
- human immunodeficiency virus type I enhancer binding protein 2
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year
1992-10-21
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Entrez gene record
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Pubmed identfication
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Classification
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VEGA ID
Gene info
Gene info
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Identity
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Gene
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Long gene name
microRNA 1-1
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Synonyms gene
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Synonyms
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Locus
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Discovery year
2004-04-23
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Entrez gene record
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RefSeq identity
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Classification
Gene info
Gene info
Gene info
Gene info
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Identity
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Gene
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Long gene name
microRNA 101-1
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Synonyms gene
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Synonyms
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Locus
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Discovery year
2004-04-23
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Entrez gene record
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RefSeq identity
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Classification
Gene info
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Identity
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Gene
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Long gene name
microRNA 1289-1
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Synonyms gene
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Synonyms
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Locus
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Discovery year
2008-11-03
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Entrez gene record
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RefSeq identity
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Classification
Gene info
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Identity
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Gene
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Long gene name
microRNA 16-1
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Synonyms gene
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Synonyms
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Locus
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Discovery year
2004-04-23
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Entrez gene record
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RefSeq identity
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Classification
MeSH Data
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Name
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Concept
Scope note:
Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
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Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
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Qualifiers
ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
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