UGPase, UDP-glucose pyrophosphorylase (cytoplasm marker) (Hordeum vulgare)

  • Catalog number
    AS14 2813
  • Price
    Please ask
  • Size
    50 µl
  • Available ordering format
    Lyophilized
  • Immunogen
    His-tagged, full length Hordeum vulgare UGPase, overexpressed and purified from E.coli, UniProt: Q43772.1
  • Raised in
    Rabbit
  • Clonality
    Polyclonal
  • Clone
    Polyclonal
  • Purification
    Serum
  • How to reconstitute
    For reconstitution add 50 µl of sterile water.
  • Storage condition
    store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
  • Verified applications
    western blot (WB)
  • Connected products
    For cytoplasmic marker for Chlamydomonas reinhardtii - please check NAB1AS05 086 | anti-UGPase | UDP-glucose pyrophosphorylase (cytoplasm marker), rabbit antibodycollection of antibodies to carbohydrate metabolismrecommended secondary antibodyPlant protein extraction buffer Secondary antibodies
  • Recommended dilutions for use
    1: 10 000 ECL (WB)
  • Molecular weight expected аpparent
    52 kDa
  • Verified reactivity
    Arabidopsis thaliana, Hordeum vulgare, Zea mays
  • Possible reactivity
    Bambusa oldhamii, Brassica pekinensis, Brassica rapa, Capsicum annuum, Cucumis sativus, Dendrobium catenatum, Dendrocalamus sinicus, Glycine max, Gossipium hirsutum, Lycopersicum esculentum, Lycopersicum chilense, Marchantia polymorpha, Oryza sativa, Picea glauca, Populus sp., Solanum tuberosum, Populus tremula, Ricinus communis, Saccharum officinarum, Vitis vinifera, for more species, please inquire
  • No reactivity
    no confirmed exceptions from predicted reactivity known in the moment
  • Supplementary information
    This antibody is also recognizing recombinant UGPase, below 0.5 pmol.
  • References
    Kleczkowski LA & Decker DD (2015) Sugar activation for production of nucleotide sugars as substrates for glycosyltransferases in plants. J. Appl. Glycosci. (in press).
  • Scientific context
    UDP-glucose pyrophosphorylase (UGPase, UDPGP) E.C=2.7.7.9. is a key enzyme of synthesis of sucrose, cellulose and other saccharides. There are two cytoplasmic isoforms of UGPase-A (which share 94 % identity on amino acid level) and one chloroplastic UGPase-B isoform in Arabidopsis thaliana which share ca. 10-11 % of identity (Kleczkowski et al. 2011). Alternative name: UTP--glucose-1-phosphate uridylyltransferase.
  • Notes
    cellular [compartment marker] of cytoplasm, UGPse is a cytoplasmic protein Martz et al. (2002)
  • Protein number
    Q43772
  • TAIR number
    AT3G03250
  • Gene target
  • Gene symbol
    GBGT1
  • Short name
    UGPase, UDP-glucose pyrophosphorylase (cytoplasm ) (Hordeum vulgare)
  • Technique
    Component cell markers and DNA bp. markers are genetic markers er genes or DNA sequences with a known location on a chromosome that can be used to identify individuals or species. Protein markers in kDA.
  • Alternative name
    UGPase, UDP-glucose pyrophosphorylase (cytoplasm detection labelled) (Hordeum vulgare)
  • Tissue
    cytoplasm
Gene info
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