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Available ordering format
Lyophilized
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Immunogen
His-tagged, full length Hordeum vulgare UGPase, overexpressed and purified from E.coli, UniProt: Q43772.1
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Raised in
Rabbit
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Clonality
Polyclonal
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Clone
Polyclonal
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Purification
Serum
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How to reconstitute
For reconstitution add 50 µl of sterile water.
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Storage condition
store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
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Verified applications
western blot (WB)
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Connected products
For cytoplasmic marker for Chlamydomonas reinhardtii - please check NAB1AS05 086 | anti-UGPase | UDP-glucose pyrophosphorylase (cytoplasm marker), rabbit antibodycollection of antibodies to carbohydrate metabolismrecommended secondary antibodyPlant protein extraction buffer Secondary antibodies
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Recommended dilutions for use
1: 10 000 ECL (WB)
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Molecular weight expected аpparent
52 kDa
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Verified reactivity
Arabidopsis thaliana, Hordeum vulgare, Zea mays
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Possible reactivity
Bambusa oldhamii, Brassica pekinensis, Brassica rapa, Capsicum annuum, Cucumis sativus, Dendrobium catenatum, Dendrocalamus sinicus, Glycine max, Gossipium hirsutum, Lycopersicum esculentum, Lycopersicum chilense, Marchantia polymorpha, Oryza sativa, Picea glauca, Populus sp., Solanum tuberosum, Populus tremula, Ricinus communis, Saccharum officinarum, Vitis vinifera, for more species, please inquire
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No reactivity
no confirmed exceptions from predicted reactivity known in the moment
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Supplementary information
This antibody is also recognizing recombinant UGPase, below 0.5 pmol.
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References
Kleczkowski LA & Decker DD (2015) Sugar activation for production of nucleotide sugars as substrates for glycosyltransferases in plants. J. Appl. Glycosci. (in press).
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Scientific context
UDP-glucose pyrophosphorylase (UGPase, UDPGP) E.C=2.7.7.9. is a key enzyme of synthesis of sucrose, cellulose and other saccharides. There are two cytoplasmic isoforms of UGPase-A (which share 94 % identity on amino acid level) and one chloroplastic UGPase-B isoform in Arabidopsis thaliana which share ca. 10-11 % of identity (Kleczkowski et al. 2011). Alternative name: UTP--glucose-1-phosphate uridylyltransferase.
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Notes
cellular [compartment marker] of cytoplasm, UGPse is a cytoplasmic protein Martz et al. (2002)
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Protein number
Q43772
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TAIR number
AT3G03250
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