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Available ordering format
Lyophilized
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Immunogen
Recombinant UGPase Q43772 overexpressed and purified from E.coli
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Raised in
Rabbit
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Clonality
Polyclonal
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Clone
Polyclonal
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Purification
Serum
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How to reconstitute
For reconstitution add 50 µl of sterile water.
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Storage condition
store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
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Verified applications
western blot (WB), immunolocalization (IL)
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Connected products
AS14 2813 | UDP-glucose pyrophosphorylase (cytoplasm marker) (Hordeum vulgare) For cytoplasmic marker for Chlamydomonas reinhardtii - please check NAB1collection of antibodies to carbohydrate metabolismrecommended secondary antibodyPlant protein extraction buffer Secondary antibodies
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Recommended dilutions for use
1: 1000 - 1: 3000 with ECL (WB), 1: 1500 (IL)
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Molecular weight expected аpparent
51.6 kDa
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Verified reactivity
Arabidopsis thaliana, Brassica rapa, Capsicum annuum, Cucumis sativus, Festuca arundinacea, Hordeum vulgare, Lycopersicum esculentum, Lycopersicum chilense, Malus x domestica Borkh. c.v. Fuji,, Marchantia polymorpha, M.vaginalis, Nicotiana tabacum, Oryza sativa, Picea glauca, Populus sp., Solanum lycopersicum, Solanum tuberosum, Solanum sogarandinu, Triticum aestivum
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Possible reactivity
Brassica pekinensis, Capsella rubella, Glycine max, Glycine soja, Gossipium hirsutum, Pinus taeda, Populus tremula, Ricinus communis, Saccharum officinarum, Zea mays, Vitis vinifera, Citrus sinensis, Jatropha curcas, Cucumis melo, Bambusa oldhamii, Eucalyptus grandis, Theobroma cacao, Gossypium hirsutum, Sorghum bicolor, Amorpha fruticosa, Brachypodium distachyon, for more species, please inquire
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No reactivity
C. merolae, diatoms
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Supplementary information
this antibody detectes 1 ng of UGPase in a western blot and reacts with both cytosolic isoforms only which have similar MW of ca. 52 kDa in Arabidopsis thaliana
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References
Liu et al. (2016). iTRAQ-based quantitative proteomic analysis reveals the role of the tonoplast in fruit senescence. J Proteomics. 2016 Sep 2;146:80-9. doi: 10.1016/j.jprot.2016.06.031. Bancel et al. (2015). Proteomic Approach to Identify Nuclear Proteins in Wheat Grain. J Proteome Res. 2015 Sep 8. Kolb et al. (2015). FYVE1 is essential for vacuole biogenesis and intracellular trafficking in Arabidopsis thaliana. Plant Physiol. 2015 Feb 19. pii: pp.114.253377. Rounis et al. (2014). Seeded and Parthenocarpic Cherry Tomato Fruits Exhibit Similar Sucrose, Glucose, and Fructose Levels, Despite Dissimilarities in UGPase and SPS Gene Expression and Enzyme Activity. J. Plant Growth Regul., July 2014. (immunolocalization)Komatsu et al. (2014). Phototropin Encoded by a Single-Copy Gene Mediates Chloroplast Photorelocation Movements in the Liverwort Marchantia polymorpha L. 1. Plant Physiol. 2014 Sep;166(1):411-27. doi: 10.1104/pp.114.245100. Epub 2014 Aug 5. Fukayama et al. (2014). Nocturnal phosphorylation of phosphoenolpyruvate carboxylase in the leaves of hygrophytic C3 monocots. Bioscience, Biotechnology and Biochemistry. Szabala et al. (2014). Accumulation of acidic SK3 dehydrins in phloem cells of cold- and drought-stressed plants of the Solanaceae. Planta, Jan 7.
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Scientific context
UDP-glucose pyrophosphorylase (UGPase, UDPGP) E.C=2.7.7.9. is a key enzyme of synthesis of sucrose, cellulose and other saccharides. There are two cytoplasmic isoforms of UGPase-A (which share 94 % identity on amino acid level) and one chloroplastic UGPase-B isoform in Arabidopsis thaliana which share ca. 10-11 % of identity (Kleczkowski et al. 2011).
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Notes
cellular [compartment marker] of cytoplasm, UGPse is a cytoplasmic protein Martz et al. (2002)
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Protein number
Q43772
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TAIR number
AT3G03250
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