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Available ordering format
Lyophilized
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Immunogen
Recombinant human S5a Proteasome subunit also known as Rpn10 P55036
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Raised in
Rabbit
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Clonality
Polyclonal
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Clone
Polyclonal
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Purification
Serum
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How to reconstitute
For reconstitution add 100 µl of sterile water.
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Storage condition
store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
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Verified applications
western blot (WB)
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Connected products
AS01 012 | anti-DS5a | Drosophila 26S proteasome subunit Rpn10Secondary antibodies
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Recommended dilutions for use
1:2000 (WB)
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Molecular weight expected аpparent
40 kDa
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Verified reactivity
human
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Possible reactivity
to be determined
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No reactivity
Drosophila melanogaster
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Supplementary information
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References
Lundgren et al. (2003). Use of RNA interference and complementation to study the function of the Drosophila and human 26S proteasome subunit S13. Mol Cel Biol 23:5320-5330.
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Scientific context
Proteasome-dependent degradation serves an essential role in the removal of a wide variety of key nuclear and cytosolic proteins. Substrates are targeted for proteolysis by the ubiquitin pathway before being degraded by the 26 S proteasome. The subunit of the proteasome, S5a, was identified to bind to polyubiquitin in vitro and thus proposed to act as a substrate recognition component. Alternative names: 26S proteasome non-ATPase regulatory subunit 4, Rpn10, multiubiquitin chain-binding protein, antisecretory factor 1, ASF, AF
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Notes
None
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Protein number
P55036
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TAIR number
Refer to NCBI
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Properties
Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
